BMRB Entry 34546

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PDB ID: 6zyg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34546
MolProbity Validation Chart

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Title:
Emfourin (M4in) from Serratia proteamaculans, M4 family peptidase inhibitor
Deposition date:
2020-07-31
Original release date:
2021-08-31
Authors:
Bozin, T.; Chukhontseva, K.; Konarev, P.; Bocharov, E.; Demidyuk, I.
Citation:

Citation: Bozin, Timur; Berdyshev, Igor; Chukhontseva, Ksenia; Karaseva, Maria; Konarev, Petr; Varizhuk, Anna; Lesovoy, Dmitry; Arseniev, Alexander; Kostrov, Sergey; Bocharov, Eduard; Demidyuk, Ilya. "NMR structure of emfourin, a novel protein metalloprotease inhibitor: Insights into the mechanism of action"  J. Biol. Chem. 299, 104585-104585 (2023).
PubMed: 36889586

Assembly members:

Assembly members:
entity_1, polymer, 113 residues, 12748.350 Da.

Natural source:

Natural source:   Common Name: Serratia proteamaculans   Taxonomy ID: 28151   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Serratia proteamaculans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pET23c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKPLPVLNQDTVIELAREGG FAFIPKLAGQRRIALADITP EQRQRLNQLLNQTLPYAQEE GQPDSPGSGDQRYFRVQISY YSQTLRSEIVLLIPETSAPQ ALVDLWKTGQVDE

Data sets:
Data typeCount
13C chemical shifts522
15N chemical shifts125
1H chemical shifts833

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 113 residues - 12748.350 Da.

1   METLYSPROLEUPROVALLEUASNGLNASP
2   THRVALILEGLULEUALAARGGLUGLYGLY
3   PHEALAPHEILEPROLYSLEUALAGLYGLN
4   ARGARGILEALALEUALAASPILETHRPRO
5   GLUGLNARGGLNARGLEUASNGLNLEULEU
6   ASNGLNTHRLEUPROTYRALAGLNGLUGLU
7   GLYGLNPROASPSERPROGLYSERGLYASP
8   GLNARGTYRPHEARGVALGLNILESERTYR
9   TYRSERGLNTHRLEUARGSERGLUILEVAL
10   LEULEUILEPROGLUTHRSERALAPROGLN
11   ALALEUVALASPLEUTRPLYSTHRGLYGLN
12   VALASPGLU

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