BMRB Entry 34556

Name: Structural evolution of the tissue-specific U2AF2 paralog and alternative splicing factor LS2
Published: 2021-10-01

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PDB ID: 7aao
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34556
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Structural evolution of the tissue-specific U2AF2 paralog and alternative splicing factor LS2

Deposition date: 2020-09-04 Original release date: 2021-10-01

Authors: Kawale, A.; Kang, H.; Sattler, M.

Citation: Kawale, A.; Kang, H.; Sattler, M.. "Structural evolution of the tissue-specific U2AF2 paralog and alternative splicing factor LS2" .

Assembly members:
entity_1, polymer, 122 residues, 13187.072 Da.

Natural source: Common Name: Fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: SISVSAMESYRSFRVPAINV AQQPAVTLPVTTIVPDSPNK IYVGGLPTCLNQDQVKELLQ SFGELKGLNLVMDTNTNLNK GFAFFEYCDPSVTDHAIAGL HGMLLGDRRLVVQRSIPGGK NA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	381
15N chemical shifts	116
1H chemical shifts	716

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 122 residues - 13187.072 Da.

1

SER

ILE

SER

VAL

SER

ALA

MET

GLU

SER

TYR

2

ARG

SER

PHE

ARG

VAL

PRO

ALA

ILE

ASN

VAL

3

ALA

GLN

PRO

ALA

VAL

THR

LEU

PRO

VAL

4

THR

ILE

VAL

PRO

ASP

SER

PRO

ASN

LYS

5

ILE

TYR

VAL

GLY

LEU

PRO

THR

CYS

LEU

6

ASN

GLN

ASP

GLN

VAL

LYS

GLU

LEU

GLN

7

SER

PHE

GLY

GLU

LEU

LYS

GLY

LEU

ASN

LEU

8

VAL

MET

ASP

THR

ASN

THR

ASN

LEU

ASN

LYS

9

GLY

PHE

ALA

PHE

GLU

TYR

CYS

ASP

PRO

10

SER

VAL

THR

ASP

HIS

ALA

ILE

ALA

GLY

LEU

11

HIS

GLY

MET

LEU

GLY

ASP

ARG

LEU

12

VAL

GLN

ARG

SER

ILE

PRO

GLY

LYS

13

ASN

ALA

Samples:

sample_1: LS2 Linker RRM2, [U-13C; U-15N], 1.0 mM; potassium phosphate 0.02 mM; sodium chloride 0.05 mM; DTT 0.002 mM

sample_conditions_1: ionic strength: 0.07 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

Sparky, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE 500 MHz
Bruker AVANCE 600 MHz
Bruker AVANCE 800 MHz
Bruker AVANCE 700 MHz
Bruker AVANCE 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts