BMRB Entry 34571

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34571
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Title:
G-quadruplex with V-shaped loop from the first repeat of KCNN4 minisatellite
Deposition date:
2020-11-02
Original release date:
2021-04-13
Authors:
Vianney, Y.; Weisz, K.
Citation:

Citation: Vianney, Y.; Weisz, K.. "First Tandem Repeat of a Potassium Channel KCNN4 Minisatellite Folds into a V-Loop G-Quadruplex Structure"  Biochemistry 60, 1337-1346 (2021).
PubMed: 33844501

Assembly members:

Assembly members:
DNA (5'-D(*GP*GP*TP*CP*TP*GP*AP*GP*GP*GP*AP*GP*AP*GP*GP*GP*GP*CP*TP*GP*GP*GP*T)-3'), polymer, 23 residues, 7298.680 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA (5'-D(*GP*GP*TP*CP*TP*GP*AP*GP*GP*GP*AP*GP*AP*GP*GP*GP*GP*CP*TP*GP*GP*GP*T)-3'): GGTCTGAGGGAGAGGGGCTG GGT

Data sets:
Data typeCount
13C chemical shifts26
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 23 residues - 7298.680 Da.

1   DGDGDTDCDTDGDADGDGDG
2   DADGDADGDGDGDGDCDTDG
3   DGDGDT

Samples:

sample_1: DNA 0.9 mM

sample_2: DNA 0.9 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HMBCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1

Software:

CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment, data analysis

TopSpin v4.0.7, Bruker Biospin - processing

X-PLOR NIH v3.0.3, Schwieters, Kuszewski, Tjandra and Clore - refinement

Amber v16, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz