BMRB Entry 34573

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PDB ID: 7avb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34573
MolProbity Validation Chart

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Title:
Solution structure of the fluorogen-activating protein FAST in the apo state
Deposition date:
2020-11-05
Original release date:
2021-04-23
Authors:
Mineev, K.; Goncharuk, S.; Baranov, M.
Citation:

Citation: Mineev, K.; Goncharuk, S.; Goncharuk, M.; Povarova, N.; Sokolov, A.; Baleeva, N.; Smirnov, A.; Myasnyanko, I.; Ruchkin, D.; Bukhdruker, S.; Remeeva, A.; Mishin, A.; Borshchevskiy, V.; Gordeliy, V.; Arseniev, A.; Gorbachev, D.; Gavrikov, A.; Mishin, A.; Baranov, M.. "NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids"  Chem. Sci. 12, 6719-6725 (2021).
PubMed: 34040747

Assembly members:

Assembly members:
entity_1, polymer, 138 residues, 15249.174 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: synthetic construct

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEHVAFGSEDIENTLAKMDD GQLDGLAFGAIQLDGDGNIL QYNAAEGDITGRDPKQVIGK NFFKDVAPGTDSPEFYGKFK EGVASGNLNTMFEWMIPTSR GPTKVKVHMKKALSGDSYWV FVKRVKLAAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts552
15N chemical shifts118
1H chemical shifts904

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 138 residues - 15249.174 Da.

1   METGLUHISVALALAPHEGLYSERGLUASP
2   ILEGLUASNTHRLEUALALYSMETASPASP
3   GLYGLNLEUASPGLYLEUALAPHEGLYALA
4   ILEGLNLEUASPGLYASPGLYASNILELEU
5   GLNTYRASNALAALAGLUGLYASPILETHR
6   GLYARGASPPROLYSGLNVALILEGLYLYS
7   ASNPHEPHELYSASPVALALAPROGLYTHR
8   ASPSERPROGLUPHETYRGLYLYSPHELYS
9   GLUGLYVALALASERGLYASNLEUASNTHR
10   METPHEGLUTRPMETILEPROTHRSERARG
11   GLYPROTHRLYSVALLYSVALHISMETLYS
12   LYSALALEUSERGLYASPSERTYRTRPVAL
13   PHEVALLYSARGVALLYSLEUALAALAALA
14   LEUGLUHISHISHISHISHISHIS

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