BMRB Entry 34598
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34598
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Tick salivary protein BSAP1 PubMed: 34118237
Deposition date: 2021-02-03 Original release date: 2021-06-18
Authors: Denisov, S.; Ippel, J.; Castoldi, E.; Hackeng, T.; Dijkgraaf, I.
Citation: Denisov, Stepan; Ippel, Johannes; Castoldi, Elisabetta; Mans, Ben; Hackeng, Tilman; Dijkgraaf, Ingrid. "The basic tail of the tick salivary protein Salp14 inhibits early stages of blood coagulation and the lectin complement pathway." J. Biol. Chem. 297, 100865-100865 (2021).
Assembly members:
entity_1, polymer, 86 residues, 9634.224 Da.
Natural source: Common Name: Ornithodoros savignyi Taxonomy ID: 69826 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Ornithodoros savignyi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: DSEFPCPRKQQPAGNSECSY
YCEMNGQWKLGKFQNGARCD
YNAVKDGVCNEGLCYASGDS
ASNTQNQGGSRRQENEDQGD
DEWDRK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 241 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 521 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 86 residues - 9634.224 Da.
| 1 | ASP | SER | GLU | PHE | PRO | CYS | PRO | ARG | LYS | GLN | ||||
| 2 | GLN | PRO | ALA | GLY | ASN | SER | GLU | CYS | SER | TYR | ||||
| 3 | TYR | CYS | GLU | MET | ASN | GLY | GLN | TRP | LYS | LEU | ||||
| 4 | GLY | LYS | PHE | GLN | ASN | GLY | ALA | ARG | CYS | ASP | ||||
| 5 | TYR | ASN | ALA | VAL | LYS | ASP | GLY | VAL | CYS | ASN | ||||
| 6 | GLU | GLY | LEU | CYS | TYR | ALA | SER | GLY | ASP | SER | ||||
| 7 | ALA | SER | ASN | THR | GLN | ASN | GLN | GLY | GLY | SER | ||||
| 8 | ARG | ARG | GLN | GLU | ASN | GLU | ASP | GLN | GLY | ASP | ||||
| 9 | ASP | GLU | TRP | ASP | ARG | LYS |
Samples:
sample_1: tick salivary protein BSAP1, [U-13C; U-15N], 350 uM
sample_conditions_1: ionic strength: 20 mM; pH: 7.1; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY, Lee W, Tonelli M, Markley JL - chemical shift assignment
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation
NMR spectrometers:
- Bruker AVANCE III HD 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts