BMRB Entry 34613

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PDB ID: 7ny0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34613
MolProbity Validation Chart

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Title:
Solution structure of Boskar4; a de novo designed G-CSF agonist
Deposition date:
2021-03-19
Original release date:
2021-10-01
Authors:
ElGamacy, M.; Coles, M.
Citation:

Citation: Skokowa, Julia; Alvarez, Birte; Coles, Murray; Ritter, Malte; Nasri, Masoud; Haaf, Jeremy; Aghaallaei, Narges; Xu, Yun; Mir, Perihan; Krahl, Ann-Christin; Rogers, Katherine; Maksymenko, Kateryna; Bajoghli, Baubak; Welte, Karl; Lupas, Andrei; Muller, Patrick; ElGamacy, Mohammad. "A topological refactoring design strategy yields highly stable granulopoietic proteins"  Nat. Commun. 13, 2948-2948 (2022).
PubMed: 35618709

Assembly members:

Assembly members:
entity_1, polymer, 121 residues, 13014.956 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: synthetic construct

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: LAAALAEIYKGLAEYQARLK SLEGISPELGPALDALRLDM ADFATTMAQAMEEGLDSLPQ SFLLKALEQIRKIQADAAAL REKLAATYKGNDRAAAAVEI AAQLEAFLEKAYQILRHLAA A

Data sets:
Data typeCount
13C chemical shifts495
15N chemical shifts115
1H chemical shifts791

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 121 residues - 13014.956 Da.

1   LEUALAALAALALEUALAGLUILETYRLYS
2   GLYLEUALAGLUTYRGLNALAARGLEULYS
3   SERLEUGLUGLYILESERPROGLULEUGLY
4   PROALALEUASPALALEUARGLEUASPMET
5   ALAASPPHEALATHRTHRMETALAGLNALA
6   METGLUGLUGLYLEUASPSERLEUPROGLN
7   SERPHELEULEULYSALALEUGLUGLNILE
8   ARGLYSILEGLNALAASPALAALAALALEU
9   ARGGLULYSLEUALAALATHRTYRLYSGLY
10   ASNASPARGALAALAALAALAVALGLUILE
11   ALAALAGLNLEUGLUALAPHELEUGLULYS
12   ALATYRGLNILELEUARGHISLEUALAALA
13   ALA

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