BMRB Entry 34621

Name: NMR structure of the Bak transmembrane helix in DPC micelles
Published: 2021-06-22

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PDB ID: 7ofm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34621
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the Bak transmembrane helix in DPC micelles

Deposition date:

2021-05-05

Original release date:

2021-06-22

Authors:

Sperl, L.; Hagn, F.

Citation:

Citation: Sperl, L.; Ruhrnossl, F.; Schiller, A.; Haslbeck, M.; Hagn, F.. "High-resolution analysis of the conformational transition of pro-apoptotic Bak at the lipid membrane" EMBO J. 40, e107159-e107159 (2021).
PubMed: 34523144

Assembly members:

Assembly members:
entity_1, polymer, 30 residues, 3244.936 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SLGNGPILNVLVVLGVVLLG QFVVRRFFKS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
13C chemical shifts	49
15N chemical shifts	24
1H chemical shifts	24

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 30 residues - 3244.936 Da.

1	SER	LEU	GLY	ASN	GLY	PRO	ILE	LEU	ASN	VAL
2	LEU	VAL	VAL	LEU	GLY	VAL	VAL	LEU	LEU	GLY
3	GLN	PHE	VAL	VAL	ARG	ARG	PHE	PHE	LYS	SER

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 34621

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	SER	LEU	GLY	ASN	GLY	PRO	ILE	LEU	ASN	VAL
2	LEU	VAL	VAL	LEU	GLY	VAL	VAL	LEU	LEU	GLY
3	GLN	PHE	VAL	VAL	ARG	ARG	PHE	PHE	LYS	SER

1	SER	LEU	GLY	ASN	GLY	PRO	ILE	LEU	ASN	VAL
2	LEU	VAL	VAL	LEU	GLY	VAL	VAL	LEU	LEU	GLY
3	GLN	PHE	VAL	VAL	ARG	ARG	PHE	PHE	LYS	SER

1	SER	LEU	GLY	ASN	GLY	PRO	ILE	LEU	ASN	VAL
2	LEU	VAL	VAL	LEU	GLY	VAL	VAL	LEU	LEU	GLY
3	GLN	PHE	VAL	VAL	ARG	ARG	PHE	PHE	LYS	SER