BMRB Entry 34629

Name: EB1 bound to MACF peptide
Published: 2022-05-26

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PDB ID: 7olg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34629
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

EB1 bound to MACF peptide

Deposition date:

2021-05-20

Original release date:

2022-05-26

Authors:

Almeida, T.; Barsukov, I.

Citation:

Citation: Almeida, T.; Barsukov, I.. "EB1 bound to MACF peptide" .

Assembly members:

Assembly members:
Microtubule-associated protein RP/EB family member 1, polymer, 70 residues, 8049.979 Da.
11MACF, polymer, 11 residues, 1283.561 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Microtubule-associated protein RP/EB family member 1: DEAAELMQQVKVLKLTVEDL EKERDFYFGKLRNIELICQE NEGENDPVLQRIVDILYATD EGFVIPDEGG
11MACF: KPSKIPTPQRK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	239
15N chemical shifts	72
1H chemical shifts	538

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1
2	unit_2	1
3	unit_3	2
4	unit_4	2

Entities:

Entity 1, unit_1 70 residues - 8049.979 Da.

1	ASP	GLU	ALA	ALA	GLU	LEU	MET	GLN	GLN	VAL
2	LYS	VAL	LEU	LYS	LEU	THR	VAL	GLU	ASP	LEU
3	GLU	LYS	GLU	ARG	ASP	PHE	TYR	PHE	GLY	LYS
4	LEU	ARG	ASN	ILE	GLU	LEU	ILE	CYS	GLN	GLU
5	ASN	GLU	GLY	GLU	ASN	ASP	PRO	VAL	LEU	GLN
6	ARG	ILE	VAL	ASP	ILE	LEU	TYR	ALA	THR	ASP
7	GLU	GLY	PHE	VAL	ILE	PRO	ASP	GLU	GLY	GLY

Entity 2, unit_3 11 residues - 1283.561 Da.

1

LYS

PRO

SER

LYS

ILE

PRO

THR

PRO

GLN

ARG

2

LYS

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 34629

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	ASP	GLU	ALA	ALA	GLU	LEU	MET	GLN	GLN	VAL
2	LYS	VAL	LEU	LYS	LEU	THR	VAL	GLU	ASP	LEU
3	GLU	LYS	GLU	ARG	ASP	PHE	TYR	PHE	GLY	LYS
4	LEU	ARG	ASN	ILE	GLU	LEU	ILE	CYS	GLN	GLU
5	ASN	GLU	GLY	GLU	ASN	ASP	PRO	VAL	LEU	GLN
6	ARG	ILE	VAL	ASP	ILE	LEU	TYR	ALA	THR	ASP
7	GLU	GLY	PHE	VAL	ILE	PRO	ASP	GLU	GLY	GLY

1	ASP	GLU	ALA	ALA	GLU	LEU	MET	GLN	GLN	VAL
2	LYS	VAL	LEU	LYS	LEU	THR	VAL	GLU	ASP	LEU
3	GLU	LYS	GLU	ARG	ASP	PHE	TYR	PHE	GLY	LYS
4	LEU	ARG	ASN	ILE	GLU	LEU	ILE	CYS	GLN	GLU
5	ASN	GLU	GLY	GLU	ASN	ASP	PRO	VAL	LEU	GLN
6	ARG	ILE	VAL	ASP	ILE	LEU	TYR	ALA	THR	ASP
7	GLU	GLY	PHE	VAL	ILE	PRO	ASP	GLU	GLY	GLY

1	ASP	GLU	ALA	ALA	GLU	LEU	MET	GLN	GLN	VAL
2	LYS	VAL	LEU	LYS	LEU	THR	VAL	GLU	ASP	LEU
3	GLU	LYS	GLU	ARG	ASP	PHE	TYR	PHE	GLY	LYS
4	LEU	ARG	ASN	ILE	GLU	LEU	ILE	CYS	GLN	GLU
5	ASN	GLU	GLY	GLU	ASN	ASP	PRO	VAL	LEU	GLN
6	ARG	ILE	VAL	ASP	ILE	LEU	TYR	ALA	THR	ASP
7	GLU	GLY	PHE	VAL	ILE	PRO	ASP	GLU	GLY	GLY