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Biological Magnetic Resonance Data Bank


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BMRB Entry 34640

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34640
MolProbity Validation Chart

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Title: Solution structure of human interleukin-9

Deposition date: 2021-06-22 Original release date: 2023-01-02

Authors: Savvides, S.; Tripsianes, K.; De Vos, T.; Papageorgiou, A.; Evangelidis, T.

Citation: De Vos, T.; Godar, M.; Bick, F.; Papageorgiou, A.; Evangelidis, T.; Markovic, I.; Mortier, E.; Dumoutier, L.; Tripsianes, K.; Blanchetot, C.; Savvides, S.. "Structural basis of the activation and antagonism of the IL-9 signaling complex."  .

Assembly members:
entity_1, polymer, 130 residues, 14551.010 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):
entity_1: GSHMQGCPTLAGILDINFLI NKMQEDPASKCHCSANVTSC LCLGIPSDNCTRPCFSERLS QMTNTTMQTRYPLIFSRVKK SVEVLKNNKCPYFSCEQPCN QTTAGNALTFLKSLLEIFQK EKMRGMRGKI

Data sets:
Data typeCount
13C chemical shifts405
15N chemical shifts126
1H chemical shifts848

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 130 residues - 14551.010 Da.

1   GLYSERHISMETGLNGLYCYSPROTHRLEU
2   ALAGLYILELEUASPILEASNPHELEUILE
3   ASNLYSMETGLNGLUASPPROALASERLYS
4   CYSHISCYSSERALAASNVALTHRSERCYS
5   LEUCYSLEUGLYILEPROSERASPASNCYS
6   THRARGPROCYSPHESERGLUARGLEUSER
7   GLNMETTHRASNTHRTHRMETGLNTHRARG
8   TYRPROLEUILEPHESERARGVALLYSLYS
9   SERVALGLUVALLEULYSASNASNLYSCYS
10   PROTYRPHESERCYSGLUGLNPROCYSASN
11   GLNTHRTHRALAGLYASNALALEUTHRPHE
12   LEULYSSERLEULEUGLUILEPHEGLNLYS
13   GLULYSMETARGGLYMETARGGLYLYSILE

Samples:

sample_1: Interleukin-9, [U-13C; U-15N], 60 mg/mL

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
4D HC(CC TOCSY(CO))NHsample_1isotropicsample_conditions_1
4D 13C,15N edited HMQC-NOESY-HSQCsample_1isotropicsample_conditions_1
4D 13C,13C edited HMQC-NOESY-HSQCsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

4D-CHAINS, Evangelidis and Tripsianes - chemical shift assignment

Sparky, Goddard - peak picking

NMR spectrometers:

  • Bruker AVANCE III 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts