BMRB Entry 34643
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34643
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Title: Solution structure of the human SF3A1 ubiquitin-like domain PubMed: 35101980
Deposition date: 2021-06-29 Original release date: 2022-02-04
Authors: de Vries, T.; Campagne, S.; Allain, F.
Citation: de Vries, T.; Martelly, W.; Campagne, S.; Sabath, K.; Sarnowski, C.; Wong, J.; Leitner, A.; Jonas, S.; Sharma, S.; Allain, F.. "Sequence-specific RNA recognition by an RGG motif connects U1 and U2 snRNP for spliceosome assembly" Proc. Natl. Acad. Sci. U. S. A. 119, e2114092119-e2114092119 (2022).
Assembly members:
entity_1, polymer, 93 residues, 10238.928 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24b
Entity Sequences (FASTA):
entity_1: GGSPVSIKVQVPNMQDKTEW
KLNGQVLVFTLPLTDQVSVI
KVKIHEATGMPAGKQKLQYE
GIFIKDSNSLAYYNMANGAV
IHLALKERGGRKK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 409 |
15N chemical shifts | 99 |
1H chemical shifts | 684 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 93 residues - 10238.928 Da.
1 | GLY | GLY | SER | PRO | VAL | SER | ILE | LYS | VAL | GLN | ||||
2 | VAL | PRO | ASN | MET | GLN | ASP | LYS | THR | GLU | TRP | ||||
3 | LYS | LEU | ASN | GLY | GLN | VAL | LEU | VAL | PHE | THR | ||||
4 | LEU | PRO | LEU | THR | ASP | GLN | VAL | SER | VAL | ILE | ||||
5 | LYS | VAL | LYS | ILE | HIS | GLU | ALA | THR | GLY | MET | ||||
6 | PRO | ALA | GLY | LYS | GLN | LYS | LEU | GLN | TYR | GLU | ||||
7 | GLY | ILE | PHE | ILE | LYS | ASP | SER | ASN | SER | LEU | ||||
8 | ALA | TYR | TYR | ASN | MET | ALA | ASN | GLY | ALA | VAL | ||||
9 | ILE | HIS | LEU | ALA | LEU | LYS | GLU | ARG | GLY | GLY | ||||
10 | ARG | LYS | LYS |
Samples:
sample_1: SF3A1, [U-13C; U-15N], 2.0 ± 0.1 mM; Sodium phosphate 10 mM; NaCl 50 mM
sample_2: SF3A1, [U-100% 15N], 1.1 ± 0.1 mM; Sodium phosphate 10 mM; NaCl 50 mM
sample_conditions_1: ionic strength: 60 mM; pH: 6.0; pressure: 1 atm; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D 15N-1H HSQC NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 13Cali-1H HSQC NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 13Caro-1H HSQC NOESY | sample_1 | isotropic | sample_conditions_1 |
{1H}-15N heteronuclear NOE | sample_2 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - data analysis
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
ATNOS, Herrmann, Guntert and Wuthrich - peak picking
CANDID, Herrmann, Guntert and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE 900 MHz
- Bruker AVIII 700 MHz
- Bruker AVANCE NEO 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts