BMRB Entry 34671
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34671
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Title: Structure of Viscotoxin A3 from Viscum Album in the complex with DPC micelles PubMed: 34781057
Deposition date: 2021-10-01 Original release date: 2021-11-28
Authors: Paramonov, A.; Shenkarev, Z.
Citation: Paramonov, Alexander; Lyukmanova, Ekaterina; Tonevitsky, Alexander; Arseniev, Alexander; Shenkarev, Zakhar. "Spatial structure and oligomerization of viscotoxin A3 in detergent micelles: Implication for mechanisms of ion channel formation and membrane lysis" Biochem. Biophys. Res. Commun. 585, 22-28 (2021).
Assembly members:
entity_1, polymer, 46 residues, 4842.626 Da.
Natural source: Common Name: European mistletoe Taxonomy ID: 3972 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Viscum album
Experimental source: Production method: .
Entity Sequences (FASTA):
entity_1: KSCCPNTTGRNIYNACRLTG
APRPTCAKLSGCKIISGSTC
PSDYPK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 295 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 46 residues - 4842.626 Da.
| 1 | LYS | SER | CYS | CYS | PRO | ASN | THR | THR | GLY | ARG | ||||
| 2 | ASN | ILE | TYR | ASN | ALA | CYS | ARG | LEU | THR | GLY | ||||
| 3 | ALA | PRO | ARG | PRO | THR | CYS | ALA | LYS | LEU | SER | ||||
| 4 | GLY | CYS | LYS | ILE | ILE | SER | GLY | SER | THR | CYS | ||||
| 5 | PRO | SER | ASP | TYR | PRO | LYS |
Samples:
sample_1: VtA3 1.6 ± 0.1 mM; DPC, [U-100% 2H], 128 ± 1 mM
sample_2: VtA3 1.6 ± 0.1 mM; DPC, [U-100% 2H], 128 ± 1 mM
sample_conditions_1: ionic strength: 10 mM; pH: 4.0; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_2 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v3.98.13, Guntert, Mumenthaler and Wuthrich - structure calculation
XEASY v3.98.13, Bartels et al. - chemical shift assignment
CARA, Keller and Wuthrich - data analysis
XEASY, Bartels et al. - peak picking
VNMR, Varian - processing
NMR spectrometers:
- Varian UNITY 600 MHz