BMRB Entry 34675

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PDB ID: 7q4l
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34675
MolProbity Validation Chart

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Title:
The solution structure of hsDND1 RRM12 bound to CUUAUUUG RNA
Deposition date:
2021-10-31
Original release date:
2022-04-29
Authors:
Duszczyk, M.; Allain, F.
Citation:

Citation: Duszczyk, M.; Wischnewski, H.; Kazeeva, T.; Arora, R.; Loughlin, F.; von Schroetter, C.; Pradere, U.; Hall, J.; Ciaudo, C.; Allain, F.. "The solution structure of Dead End bound to AU-rich RNA reveals an unusual mode of tandem RRM-RNA recognition required for mRNA regulation"  Nat. Commun. 13, 5892-5892 (2022).
PubMed: 36202814

Assembly members:

Assembly members:
entity_1, polymer, 227 residues, 24927.939 Da.
entity_2, polymer, 8 residues, 2465.465 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMERVNPENKAALEAWVRE TGIRLVQVNGQRKYGGPPPG WVGSPPPAGSEVFIGRLPQD VYEHQLIPLFQRVGRLYEFR LMMTFSGLNRGFAYARYSSR RGAQAAIATLHNHPLRPSCP LLVCRSTEKCELSVDGLPPN LTRSALLLALQPLGPGLQEA RLLPSPGPAPGQIALLKFSS HRAAAMAKKALVEGQSHLCG EQVAVEWLKPDLKQRLRQQL VGPFLRS
entity_2: CUUAUUUG

Data sets:
Data typeCount
13C chemical shifts637
15N chemical shifts202
1H chemical shifts1461

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 227 residues - 24927.939 Da.

1   GLYALAMETGLUARGVALASNPROGLUASN
2   LYSALAALALEUGLUALATRPVALARGGLU
3   THRGLYILEARGLEUVALGLNVALASNGLY
4   GLNARGLYSTYRGLYGLYPROPROPROGLY
5   TRPVALGLYSERPROPROPROALAGLYSER
6   GLUVALPHEILEGLYARGLEUPROGLNASP
7   VALTYRGLUHISGLNLEUILEPROLEUPHE
8   GLNARGVALGLYARGLEUTYRGLUPHEARG
9   LEUMETMETTHRPHESERGLYLEUASNARG
10   GLYPHEALATYRALAARGTYRSERSERARG
11   ARGGLYALAGLNALAALAILEALATHRLEU
12   HISASNHISPROLEUARGPROSERCYSPRO
13   LEULEUVALCYSARGSERTHRGLULYSCYS
14   GLULEUSERVALASPGLYLEUPROPROASN
15   LEUTHRARGSERALALEULEULEUALALEU
16   GLNPROLEUGLYPROGLYLEUGLNGLUALA
17   ARGLEULEUPROSERPROGLYPROALAPRO
18   GLYGLNILEALALEULEULYSPHESERSER
19   HISARGALAALAALAMETALALYSLYSALA
20   LEUVALGLUGLYGLNSERHISLEUCYSGLY
21   GLUGLNVALALAVALGLUTRPLEULYSPRO
22   ASPLEULYSGLNARGLEUARGGLNGLNLEU
23   VALGLYPROPHELEUARGSER

Entity 2, unit_2 8 residues - 2465.465 Da.

1   CUUAUUUG

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