BMRB Entry 34686
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34686
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Refined structure of the T193A mutant in the C-terminal domain of DNAJB6b PubMed: 35247211
Deposition date: 2021-11-21 Original release date: 2022-03-28
Authors: Karamanos, T.; Cawood, E.
Citation: Cawood, E.; Clore, G.; Karamanos, T.. "Microsecond Backbone Motions Modulate the Oligomerization of the DNAJB6 Chaperone." Angew. Chem. Int. Ed. Engl. 61, e202116403-e202116403 (2022).
Assembly members:
entity_1, polymer, 57 residues, 6444.378 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)
Entity Sequences (FASTA):
entity_1: MGNFKSISASTKMVNGRKIT
TKRIVENGQERVEVEEDGQL
KSLTINGKEQLLRLDNK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 101 |
| 15N chemical shifts | 50 |
| 1H chemical shifts | 53 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 57 residues - 6444.378 Da.
| 1 | MET | GLY | ASN | PHE | LYS | SER | ILE | SER | ALA | SER | ||||
| 2 | THR | LYS | MET | VAL | ASN | GLY | ARG | LYS | ILE | THR | ||||
| 3 | THR | LYS | ARG | ILE | VAL | GLU | ASN | GLY | GLN | GLU | ||||
| 4 | ARG | VAL | GLU | VAL | GLU | GLU | ASP | GLY | GLN | LEU | ||||
| 5 | LYS | SER | LEU | THR | ILE | ASN | GLY | LYS | GLU | GLN | ||||
| 6 | LEU | LEU | ARG | LEU | ASP | ASN | LYS |
Samples:
sample_1: DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN, [U-13C; U-15N], 0.2 mM; sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.02 % v/v
sample_conditions_1: ionic strength: 87 mM; pH: 6.7; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-13C NOESY HCH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY CCH | sample_1 | isotropic | sample_conditions_1 |
| JHNHA ARTSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CcpNmr Analysis, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz