BMRB Entry 34713
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34713
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NMR-STAR v3 text file.
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Title: Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) G22L and G26L double mutant TMD PubMed: 36470941
Deposition date: 2022-02-22 Original release date: 2023-01-02
Authors: Silber, M.; Muhle-Goll, C.
Citation: Papadopoulou, Alkmini; Stelzer, Walter; Silber, Mara; Schlosser, Christine; Spitz, Charlotte; Haug-Kroper, Martina; Straub, Tobias; Muller, Stephan; Lichtenthaler, Stefan; Muhle-Goll, Claudia; Langosch, Dieter; Fluhrer, Regina. "Helical stability of the GnTV transmembrane domain impacts on SPPL3 dependent cleavage" Sci. Rep. 12, 20987-20987 (2022).
Assembly members:
entity_1, polymer, 29 residues, 3663.636 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: KKKLGFFLVTFLFIWLMMLL
HFTIQQRKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 99 |
| 15N chemical shifts | 29 |
| 1H chemical shifts | 196 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 29 residues - 3663.636 Da.
| 1 | LYS | LYS | LYS | LEU | GLY | PHE | PHE | LEU | VAL | THR | ||||
| 2 | PHE | LEU | PHE | ILE | TRP | LEU | MET | MET | LEU | LEU | ||||
| 3 | HIS | PHE | THR | ILE | GLN | GLN | ARG | LYS | LYS |
Samples:
sample_1: GNTV_G22LG26L 500 uM
sample_conditions_1: ionic strength: 1 Not defined; pH: 7.0; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - refinement
ARIA, Linge, O'Donoghue and Nilges - structure calculation
CcpNmr Analysis, Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts