BMRB Entry 34754

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PDB ID: 8au4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34754
MolProbity Validation Chart

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Title:
Structural insights reveal a heterotetramer between oncogenic K-Ras4BG12V and Rgl2, a RalA/B activator
Deposition date:
2022-08-25
Original release date:
2023-10-31
Authors:
Tariq, M.; Ikeya, T.; Togashi, N.; Fairall, L.; Alejo, C.; Kamei, S.; Alonso, B.; Campillo, M.; Hudson, A.; Ito, Y.; Schwabe, J.; Dominguez, C.; Tanaka, K.
Citation:

Citation: Tariq, M.; Ikeya, T.; Togashi, N.; Fairall, L.; Kamei, S.; Mayooramurugan, S.; Abbott, L.; Hasan, A.; Bueno-Alejo, C.; Sukegawa, S.; Romartinez-Alonso, B.; Muro Campillo, M.; Hudson, A.; Ito, Y.; Schwabe, J.; Dominguez, C.; Tanaka, K.. "Structural insights into the complex of oncogenic KRas4B G12V and Rgl2, a RalA/B activator."  Life Sci. Alliance 7, e202302080-e202302080 (2023).
PubMed: 37833074

Assembly members:

Assembly members:
entity_1, polymer, 100 residues, 10972.406 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMGPGASDCRIIRVQMELGE DGSVYKSILVTSQDKAPSVI SRVLKKNNRDSAVASEYELV QLLPGERELTIPASANVFYA MDGASHDFLLRQRRRSSTAT

Data sets:
Data typeCount
13C chemical shifts415
15N chemical shifts100
1H chemical shifts672

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 100 residues - 10972.406 Da.

1   SERMETGLYPROGLYALASERASPCYSARG
2   ILEILEARGVALGLNMETGLULEUGLYGLU
3   ASPGLYSERVALTYRLYSSERILELEUVAL
4   THRSERGLNASPLYSALAPROSERVALILE
5   SERARGVALLEULYSLYSASNASNARGASP
6   SERALAVALALASERGLUTYRGLULEUVAL
7   GLNLEULEUPROGLYGLUARGGLULEUTHR
8   ILEPROALASERALAASNVALPHETYRALA
9   METASPGLYALASERHISASPPHELEULEU
10   ARGGLNARGARGARGSERSERTHRALATHR

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