BMRB Entry 34781

Click here to enlarge.

PDB ID: 8c18
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34781
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution structure of carotenoid-binding protein AstaPo1 in complex with astaxanthin
Deposition date:
2022-12-20
Original release date:
2023-03-29
Authors:
Kornilov, F.; Savitskaya, A.; Slonimskiy, Y.; Goncharuk, S.; Sluchanko, N.; Mineev, K.
Citation:

Citation: Kornilov, F.; Slonimsky, Y.; Lunegova, D.; Egorkin, N.; Savitskaya, A.; Kleymenov, S.; Maksimov, E.; Goncharuk, S.; Mineev, K.; Sluchanko, N.. "Structural basis for the ligand promiscuity of the neofunctionalized, carotenoid-binding fasciclin domain protein AstaP"  Commun. Biol. 6, 471-471 (2023).
PubMed: 37117801

Assembly members:

Assembly members:
entity_1, polymer, 207 residues, 21634.139 Da.
entity_AXT, non-polymer, 596.838 Da.

Natural source:

Natural source:   Common Name: green algae   Taxonomy ID: 2833457   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Coelastrella astaxanthina

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPHMATPKANATTAKPASTT STPVYATLSNAVTAGAAAPQ LTTLFAAVRAANVTGALTAN TTWTILAPTNDAFAKRLAKL NLTADAVLKNKDLLVKILSY HVIPSGAVYSKALKDNATVA TALKDASVTVRLYQGKVMFK GPVNKAQVTVADIKAGGSVI HVINDVLLPPGVVSDAVAKQ WKAEWEAMKAEKKVAPKATT GRRFLLF

Data sets:
Data typeCount
13C chemical shifts851
15N chemical shifts178
1H chemical shifts1397

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 207 residues - 21634.139 Da.

1   GLYPROHISMETALATHRPROLYSALAASN
2   ALATHRTHRALALYSPROALASERTHRTHR
3   SERTHRPROVALTYRALATHRLEUSERASN
4   ALAVALTHRALAGLYALAALAALAPROGLN
5   LEUTHRTHRLEUPHEALAALAVALARGALA
6   ALAASNVALTHRGLYALALEUTHRALAASN
7   THRTHRTRPTHRILELEUALAPROTHRASN
8   ASPALAPHEALALYSARGLEUALALYSLEU
9   ASNLEUTHRALAASPALAVALLEULYSASN
10   LYSASPLEULEUVALLYSILELEUSERTYR
11   HISVALILEPROSERGLYALAVALTYRSER
12   LYSALALEULYSASPASNALATHRVALALA
13   THRALALEULYSASPALASERVALTHRVAL
14   ARGLEUTYRGLNGLYLYSVALMETPHELYS
15   GLYPROVALASNLYSALAGLNVALTHRVAL
16   ALAASPILELYSALAGLYGLYSERVALILE
17   HISVALILEASNASPVALLEULEUPROPRO
18   GLYVALVALSERASPALAVALALALYSGLN
19   TRPLYSALAGLUTRPGLUALAMETLYSALA
20   GLULYSLYSVALALAPROLYSALATHRTHR
21   GLYARGARGPHELEULEUPHE

Entity 2, unit_2 - C40 H52 O4 - 596.838 Da.

1   AXT

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks