BMRB Entry 34784
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34784
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Title: Solution structure of the RNA helix formed by the 5'-end of U1 snRNA and an A-1 bulged 5'-splice site in complex with SMN-CY
Deposition date: 2023-02-02 Original release date: 2024-02-13
Authors: Malard, F.; Marquevielle, J.; Campagne, S.
Citation: Malard, F.; Marquevielle, J.; Campagne, S.. "The diversity of splicing modifiers acting on A-1 bulged 5'-splice sites reveals rules to guide rational design" .
Assembly members:
entity_1, polymer, 11 residues, 3428.074 Da.
entity_2, polymer, 11 residues, 3547.162 Da.
entity_3, non-polymer, 382.435 Da.
Natural source: Common Name: not available Taxonomy ID: 32630 Superkingdom: not available Kingdom: not available Genus/species: synthetic construct
Experimental source: Production method: recombinant technology Host organism: synthetic construct
Entity Sequences (FASTA):
entity_1: AUACXXACCUG
entity_2: GGAGUAAGUCU
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 147 |
1H chemical shifts | 194 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | unit_1 | 1 |
2 | unit_2 | 2 |
3 | unit_3 | 3 |
Entities:
Entity 1, unit_1 11 residues - 3428.074 Da.
1 | A | U | A | C | PSU | PSU | A | C | C | U | ||||
2 | G |
Entity 2, unit_2 11 residues - 3547.162 Da.
1 | G | G | A | G | U | A | A | G | U | C | ||||
2 | U |
Entity 3, unit_3 - 382.435 Da.
1 | ULR |
Samples:
sample_1: RNA helix, unlableled, 1 ± 0.005 mM; RNA helix, unlableled, 1 ± 0.005 mM; SMN-CY, unlabeld, 1.5 ± 0.005 mM; MES, deuterated, 10 ± 0.001 mM; sodium chloride, unlabeld, 50 ± 0.001 mM
sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 293 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
Amber v22, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - data analysis, refinement
CYANA v3.98, Guntert, Mumenthaler and Wuthrich - structure calculation
CARA, Keller and Wuthrich - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker AVIII 900 MHz
- Bruker AVIII 700 MHz