BMRB Entry 34908

Name: Solution Structure of cAMP-dependent Protein Kinase RII-alpha Subunit Dimerization and Docking Domain Complex with Microtubule Associated Protein 2c (84-111)
Published: 2024-10-15

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PDB ID: 8s8o
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34908
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of cAMP-dependent Protein Kinase RII-alpha Subunit Dimerization and Docking Domain Complex with Microtubule Associated Protein 2c (84-111)

Deposition date:

2024-03-06

Original release date:

2024-10-15

Authors:

Bartosik, V.; Lanikova, A.; Janackova, Z.; Padrta, P.; Zidek, L.

Citation:

Citation: Bartosik, V.; Plucarova, J.; Lanikova, A.; Janackova, Z.; Padrta, P.; Jansen, S.; Varecka, V.; Gruber, T.; Feller, S.; Zidek, L.. "Structural basis of binding the unique N-terminal domain of microtubule-associated protein 2c to proteins regulating kinases of signaling pathways." J. Biol. Chem. 300, 107551-107551 (2024).
PubMed: 39002671

Assembly members:

Assembly members:
entity_1, polymer, 52 residues, 5789.617 Da.
entity_2, polymer, 28 residues, 3015.349 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3) Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGMSHIQIPPGLTELLQG YTVEVLRQQPPDLVEFAVEY FTRLREARAPAS
entity_2: RETAEEVSARIVQVVTAEAV AVLKGEQE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
13C chemical shifts	249
15N chemical shifts	90
1H chemical shifts	575

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1
2	unit_2	1
3	unit_3	2

Entities:

Entity 1, unit_1 52 residues - 5789.617 Da.

1

GLY

ALA

MET

GLY

MET

SER

HIS

ILE

GLN

ILE

2

PRO

GLY

LEU

THR

GLU

LEU

GLN

GLY

3

TYR

THR

VAL

GLU

VAL

LEU

ARG

GLN

PRO

4

PRO

ASP

LEU

VAL

GLU

PHE

ALA

VAL

GLU

TYR

5

PHE

THR

ARG

LEU

ARG

GLU

ALA

ARG

ALA

PRO

6

ALA

SER

Entity 2, unit_3 28 residues - 3015.349 Da.

1

ARG

GLU

THR

ALA

GLU

VAL

SER

ALA

ARG

2

ILE

VAL

GLN

VAL

THR

ALA

GLU

ALA

VAL

3

ALA

VAL

LEU

LYS

GLY

GLU

GLN

GLU

Samples:

sample_1: Microtubule associated protein 2c, [U-13C; U-15N], 0.5 mM; cAMP-dependent protein kinase type II-alpha regulatory subunit 2 mM; MOPS 50 mM; sodium chloride 100 mM; D2O 10 % v/v; sodium azide 17 mM

sample_2: Microtubule associated protein 2c 0.56 mM; cAMP-dependent protein kinase type II-alpha regulatory subunit, [U-13C; U-15N], 1.12 mM; MOPS 50 mM; sodium chloride 100 mM; D2O 10 % v/v; sodium azide 10 mM

sample_3: Microtubule associated protein 2c, [U-15N], 0.1 mM; cAMP-dependent protein kinase type II-alpha regulatory subunit 0.4 mM; MOPS 50 mM; sodium chloride 100 mM; D2O 8 % v/v; Pf1 phage 40 mg/mL

sample_4: Microtubule associated protein 2c, [U-15N], 0.23 mM; cAMP-dependent protein kinase type II-alpha regulatory subunit 0.92 mM; MOPS 50 mM; sodium chloride 100 mM; D2O 7.5 % v/v

sample_5: Microtubule associated protein 2c, [I,V-13C; I,L,V-15N], 0.45 mM; cAMP-dependent protein kinase type II-alpha regulatory subunit 1.8 mM; MOPS 50 mM; sodium chloride 100 mM; D2O 7.5 % v/v

sample_conditions_1: ionic strength: 100 mM; pH: 6.9; pressure: 1 bar; temperature: 300.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY filtered	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic filtered	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY filtered	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic filtered	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_4	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	anisotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC IPAP	sample_4	isotropic	sample_conditions_1
2D 1H-15N HSQC IPAP	sample_3	anisotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_5	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_5	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_5	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks