BMRB Entry 36059
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR36059
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Title: Solution structure of musashi1 RBD2 in complex with RNA PubMed: 28753936
Deposition date: 2017-02-09 Original release date: 2017-12-11
Authors: Iwaoka, R.; Nagata, T.; Tsuda, K.; Imai, T.; Okano, H.; Kobayashi, N.; Katahira, M.
Citation: Iwaoka, R.; Nagata, T.; Tsuda, K.; Imai, T.; Okano, H.; Kobayashi, N.; Katahira, M.. "Structural Insight into the Recognition of r(UAG) by Musashi-1 RBD2, and Construction of a Model of Musashi-1 RBD1-2 Bound to the Minimum Target RNA" Molecules 22, 1207-1207 (2017).
Assembly members:
entity_1, polymer, 96 residues, 10945.506 Da.
entity_2, polymer, 5 residues, 1586.992 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
entity_1: GSHMKKIFVGGLSVNTTVED
VKHYFEQFGKVDDAMLMFDK
TTNRHRGFGFVTFESEDIVE
KVCEIHFHEINNKMVECKKA
QPKEVMSPTGSARGRS
entity_2: GUAGU
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 416 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 614 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 96 residues - 10945.506 Da.
| 1 | GLY | SER | HIS | MET | LYS | LYS | ILE | PHE | VAL | GLY | ||||
| 2 | GLY | LEU | SER | VAL | ASN | THR | THR | VAL | GLU | ASP | ||||
| 3 | VAL | LYS | HIS | TYR | PHE | GLU | GLN | PHE | GLY | LYS | ||||
| 4 | VAL | ASP | ASP | ALA | MET | LEU | MET | PHE | ASP | LYS | ||||
| 5 | THR | THR | ASN | ARG | HIS | ARG | GLY | PHE | GLY | PHE | ||||
| 6 | VAL | THR | PHE | GLU | SER | GLU | ASP | ILE | VAL | GLU | ||||
| 7 | LYS | VAL | CYS | GLU | ILE | HIS | PHE | HIS | GLU | ILE | ||||
| 8 | ASN | ASN | LYS | MET | VAL | GLU | CYS | LYS | LYS | ALA | ||||
| 9 | GLN | PRO | LYS | GLU | VAL | MET | SER | PRO | THR | GLY | ||||
| 10 | SER | ALA | ARG | GLY | ARG | SER |
Entity 2, entity_2 5 residues - 1586.992 Da.
| 1 | G | U | A | G | U |
Samples:
sample_1: Msi1 RBD2, [U-100% 13C; U-100% 15N], 300 uM; RNA (5'-R(*GP*UP*AP*GP*U)-3') 300 uM; MES 20 mM; sodium chloride 100 mM; H2O 95%; D2O, [U-2H], 5%
sample_conditions_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
MagRO, Kobayashi - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker AvanceIII 950 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts