BMRB Entry 36157
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36157
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Title: Nukacin ISK-1 in active state PubMed: 30272026
Deposition date: 2018-01-19 Original release date: 2019-06-05
Authors: Kohda, D.; Fujinami, D.
Citation: Fujinami, Daisuke; Mahin, Abdullah-Al; Elsayed, Khaled M; Islam, Mohammad R; Nagao, Jun-Ichi; Roy, Urmi; Momin, Sabrina; Zendo, Takeshi; Kohda, Daisuke; Sonomoto, Kenji. "The lantibiotic nukacin ISK-1 exists in an equilibrium between active and inactive lipid-II binding states." Commun. Biol. 1, 150-150 (2018).
Assembly members:
Lantibiotic nukacin, polymer, 27 residues, 2970.535 Da.
Natural source: Common Name: Staphylococcus warneri Taxonomy ID: 1292 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus warneri
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Lantibiotic nukacin: KKKSGVIPXVXHDCHMNXFQ
FVFXCCS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 174 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 27 residues - 2970.535 Da.
| 1 | LYS | LYS | LYS | SER | GLY | VAL | ILE | PRO | DBB | VAL | ||||
| 2 | DAL | HIS | ASP | CYS | HIS | MET | ASN | DAL | PHE | GLN | ||||
| 3 | PHE | VAL | PHE | DBU | CYS | CYS | SER |
Samples:
sample_1: dodecylphosphocholine, [U-2H], 34 mM; glycine 10 mM; nukacin ISK-1 13 mM; H2O 90%; D2O, [U-2H], 10%
sample_conditions_1: ionic strength: 0 mM; pH: 3.5; pressure: 1 atm; temperature: 328 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert P. - refinement
NMR spectrometers:
- Bruker Avance 700 MHz