BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 36170

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR36170

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Title: NMR structure of IRD7 from Capsicum annum.   PubMed: 30229451

Deposition date: 2018-03-06 Original release date: 2019-03-25

Authors: Gartia, J.; Barnwal, R.; Chary, K.

Citation: Gartia, Janeka; Barnwal, Ravi Pratap; Anangi, Raveendra; Giri, Ashok; King, Glenn; Chary, Kandala. "."  Biomol. NMR Assign. ., .-. (2018).

Assembly members:
entity_1, polymer, 50 residues, 5363.004 Da.

Natural source:   Common Name: Bell pepper   Taxonomy ID: 4072   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Capsicum annuum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: EPICTNCCAGLKGCNYYNAD GTFICEGESDPNHPKACPKN CDPNIAYSLC

Data sets:
Data typeCount
13C chemical shifts184
15N chemical shifts50
1H chemical shifts264

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 50 residues - 5363.004 Da.

1   GLUPROILECYSTHRASNCYSCYSALAGLY
2   LEULYSGLYCYSASNTYRTYRASNALAASP
3   GLYTHRPHEILECYSGLUGLYGLUSERASP
4   PROASNHISPROLYSALACYSPROLYSASN
5   CYSASPPROASNILEALATYRSERLEUCYS

Samples:

sample_1: IRD7, [U-95% 13C; U-95% 15N], 1.0 mM; Phosphate 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1

Software:

CYANA v3.97, Guntert, Mumenthaler and Wuthrich - structure calculation

CcpNMR, CCPN - peak picking

CcpNMR v3.97, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AvanceIII 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts