BMRB Entry 36273
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36273
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Title: NMR solution structure of Roseltide rT7 PubMed: 31727740
Deposition date: 2019-07-30 Original release date: 2019-11-20
Authors: Fan, J.; Kam, A.; Loo, S.; Yang, D.; Tam, P.
Citation: Kam, A.; Loo, S.; Fan, J.; Sze, S.; Yang, D.; Tam, J.. "Roseltide rT7 is a disulfide-rich, anionic, and cell-penetrating peptide that inhibits proteasomal degradation" J. Biol. Chem. 294, 19604-19615 (2019).
Assembly members:
entity_1, polymer, 34 residues, 3707.386 Da.
Natural source: Common Name: Hibiscus sabdariffa Taxonomy ID: 183260 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Hibiscus sabdariffa
Experimental source: Production method: na Host organism: na
Entity Sequences (FASTA):
entity_1: CVSSGIVDACSECCEPDKCI
IMLPTWPPRYVCSV
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 172 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 34 residues - 3707.386 Da.
1 | CYS | VAL | SER | SER | GLY | ILE | VAL | ASP | ALA | CYS | ||||
2 | SER | GLU | CYS | CYS | GLU | PRO | ASP | LYS | CYS | ILE | ||||
3 | ILE | MET | LEU | PRO | THR | TRP | PRO | PRO | ARG | TYR | ||||
4 | VAL | CYS | SER | VAL |
Samples:
sample_1: Roseltide 1.0 ± 0.1 mM; D2O, [U-2H], 6%; H2O 94%
sample_conditions_1: ionic strength: 0 mM; pH: 4; pressure: 1 atm; temperature: 307 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H ROESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - refinement
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Sparky, Goddard - data analysis, peak picking
NMR spectrometers:
- Bruker AVANCE 800 MHz