BMRB Entry 36294

Assembly:

Entities:

Entity 1, entity_1, 1 81 residues - 8990.265 Da.

Samples:

sample_1: human Bax core domain, [U-98% 15N; U-98% 13C; U-98% 2H], 0.5 mM; DHPC 140 mM; DMPC 100 mM; MES 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: Bax a_2_a_5, [U-98% 15N; U-98% 13C], 0.6 mM; DHPC, [U-2H], 160 mM; DMPC, [U-2H], 100 mM; MES 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_3: Bax a_2_a_5, U-98% 15N;U-2H, 0.4 mM; DHPC, [U-2H], 200 mM; DMPC, [U-2H], 120 mM; MES 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_4: Bax a_2_a_5, [U-98% 15N; U-2H], 0.6 mM; DHPC, [U-2H], 160 mM; DMPC 100 mM; MES 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_5: Bax a_2_a_5, [U-98% 15N; U-15% 13C], 0.4 mM; DHPC 140 mM; DMPC 80 mM; MES 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_6: Bax a_2_a_5, [U-98% 13C], 0.4 mM; DHPC, [U-2H], 200 mM; DMPC, [U-2H], 120 mM; MES 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 305 K

Experiments:

Software:

CcpNmr Analysis, CCPN - peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

TopSpin, Bruker Biospin - collection

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

• Bruker AVANCE 600 MHz
• Bruker AVANCE 900 MHz
• Agilent DD2 800 MHz