BMRB Entry 36405

Name: Solution structure of RPB6, common subunit of RNA polymerases I, II, and III
Published: 2022-01-24

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PDB ID: 7dth
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36405
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of RPB6, common subunit of RNA polymerases I, II, and III

Deposition date:

2021-01-05

Original release date:

2022-01-24

Authors:

Okuda, M.; Nishimura, Y.

Citation:

Citation: Okuda, M.; Suwa, T.; Suzuki, H.; Yamaguchi, Y.; Nishimura, Y.. "Three human RNA polymerases interact with TFIIH via a common RPB6 subunit" Nucleic Acids Res. 50, 1-16 (2022).
PubMed: 34268577

Assembly members:

Assembly members:
DNA-directed RNA polymerases I, II, and III subunit RPABC2, polymer, 130 residues, 14773.305 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA-directed RNA polymerases I, II, and III subunit RPABC2: GSHMSDNEDNFDGDDFDDVE EDEGLDDLENAEEEGQENVE ILPSGERPQANQKRITTPYM TKYERARVLGTRALQIAMCA PVMVELEGETDPLLIAMKEL KARKIPIIIRRYLPDGSYED WGVDELIITD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
heteronucl_NOEs
- heteronuclear_noe_list_1

Data type	Count
13C chemical shifts	548
15N chemical shifts	136
1H chemical shifts	825
heteronuclear NOE values	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 130 residues - 14773.305 Da.

1	GLY	SER	HIS	MET	SER	ASP	ASN	GLU	ASP	ASN
2	PHE	ASP	GLY	ASP	ASP	PHE	ASP	ASP	VAL	GLU
3	GLU	ASP	GLU	GLY	LEU	ASP	ASP	LEU	GLU	ASN
4	ALA	GLU	GLU	GLU	GLY	GLN	GLU	ASN	VAL	GLU
5	ILE	LEU	PRO	SER	GLY	GLU	ARG	PRO	GLN	ALA
6	ASN	GLN	LYS	ARG	ILE	THR	THR	PRO	TYR	MET
7	THR	LYS	TYR	GLU	ARG	ALA	ARG	VAL	LEU	GLY
8	THR	ARG	ALA	LEU	GLN	ILE	ALA	MET	CYS	ALA
9	PRO	VAL	MET	VAL	GLU	LEU	GLU	GLY	GLU	THR
10	ASP	PRO	LEU	LEU	ILE	ALA	MET	LYS	GLU	LEU
11	LYS	ALA	ARG	LYS	ILE	PRO	ILE	ILE	ILE	ARG
12	ARG	TYR	LEU	PRO	ASP	GLY	SER	TYR	GLU	ASP
13	TRP	GLY	VAL	ASP	GLU	LEU	ILE	ILE	THR	ASP

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHANH	sample_1	isotropic	sample_conditions_1
3D HN(CO)HB	sample_1	isotropic	sample_conditions_1
3D HNHB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CG	sample_1	isotropic	sample_conditions_1
3D HNCG	sample_1	isotropic	sample_conditions_1
3D CC(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCC(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 36405

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	GLY	SER	HIS	MET	SER	ASP	ASN	GLU	ASP	ASN
2	PHE	ASP	GLY	ASP	ASP	PHE	ASP	ASP	VAL	GLU
3	GLU	ASP	GLU	GLY	LEU	ASP	ASP	LEU	GLU	ASN
4	ALA	GLU	GLU	GLU	GLY	GLN	GLU	ASN	VAL	GLU
5	ILE	LEU	PRO	SER	GLY	GLU	ARG	PRO	GLN	ALA
6	ASN	GLN	LYS	ARG	ILE	THR	THR	PRO	TYR	MET
7	THR	LYS	TYR	GLU	ARG	ALA	ARG	VAL	LEU	GLY
8	THR	ARG	ALA	LEU	GLN	ILE	ALA	MET	CYS	ALA
9	PRO	VAL	MET	VAL	GLU	LEU	GLU	GLY	GLU	THR
10	ASP	PRO	LEU	LEU	ILE	ALA	MET	LYS	GLU	LEU
11	LYS	ALA	ARG	LYS	ILE	PRO	ILE	ILE	ILE	ARG
12	ARG	TYR	LEU	PRO	ASP	GLY	SER	TYR	GLU	ASP
13	TRP	GLY	VAL	ASP	GLU	LEU	ILE	ILE	THR	ASP

1	GLY	SER	HIS	MET	SER	ASP	ASN	GLU	ASP	ASN
2	PHE	ASP	GLY	ASP	ASP	PHE	ASP	ASP	VAL	GLU
3	GLU	ASP	GLU	GLY	LEU	ASP	ASP	LEU	GLU	ASN
4	ALA	GLU	GLU	GLU	GLY	GLN	GLU	ASN	VAL	GLU
5	ILE	LEU	PRO	SER	GLY	GLU	ARG	PRO	GLN	ALA
6	ASN	GLN	LYS	ARG	ILE	THR	THR	PRO	TYR	MET
7	THR	LYS	TYR	GLU	ARG	ALA	ARG	VAL	LEU	GLY
8	THR	ARG	ALA	LEU	GLN	ILE	ALA	MET	CYS	ALA
9	PRO	VAL	MET	VAL	GLU	LEU	GLU	GLY	GLU	THR
10	ASP	PRO	LEU	LEU	ILE	ALA	MET	LYS	GLU	LEU
11	LYS	ALA	ARG	LYS	ILE	PRO	ILE	ILE	ILE	ARG
12	ARG	TYR	LEU	PRO	ASP	GLY	SER	TYR	GLU	ASP
13	TRP	GLY	VAL	ASP	GLU	LEU	ILE	ILE	THR	ASP

1	GLY	SER	HIS	MET	SER	ASP	ASN	GLU	ASP	ASN
2	PHE	ASP	GLY	ASP	ASP	PHE	ASP	ASP	VAL	GLU
3	GLU	ASP	GLU	GLY	LEU	ASP	ASP	LEU	GLU	ASN
4	ALA	GLU	GLU	GLU	GLY	GLN	GLU	ASN	VAL	GLU
5	ILE	LEU	PRO	SER	GLY	GLU	ARG	PRO	GLN	ALA
6	ASN	GLN	LYS	ARG	ILE	THR	THR	PRO	TYR	MET
7	THR	LYS	TYR	GLU	ARG	ALA	ARG	VAL	LEU	GLY
8	THR	ARG	ALA	LEU	GLN	ILE	ALA	MET	CYS	ALA
9	PRO	VAL	MET	VAL	GLU	LEU	GLU	GLY	GLU	THR
10	ASP	PRO	LEU	LEU	ILE	ALA	MET	LYS	GLU	LEU
11	LYS	ALA	ARG	LYS	ILE	PRO	ILE	ILE	ILE	ARG
12	ARG	TYR	LEU	PRO	ASP	GLY	SER	TYR	GLU	ASP
13	TRP	GLY	VAL	ASP	GLU	LEU	ILE	ILE	THR	ASP