BMRB Entry 36515

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PDB ID: 8hep
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36515
MolProbity Validation Chart

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Title:
Solution structure of the periplasmic domain of the anti-sigma factor RsgI1 from Clostridium thermocellum
Deposition date:
2022-11-08
Original release date:
2023-05-30
Authors:
Chen, C.; Feng, Y.
Citation:

Citation: Chen, C.; Feng, Y.. "Essential autoproteolysis of bacterial anti-sigma factor RsgI for signal transmembrane transduction"  .

Assembly members:

Assembly members:
Anti-sigma factor, polymer, 10 residues, 1190.390 Da.
Anti-sigma factor, polymer, 166 residues, 19081.623 Da.

Natural source:

Natural source:   Common Name: Acetivibrio thermocellus DSM 1313   Taxonomy ID: 637887   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Acetivibrio thermocellus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Anti-sigma factor: MYGYICVDIN
Anti-sigma factor: PSVELVIDETCRVLEVRPQN KDGEQLISGLELLDKNVEDV VYELINRSISFGFVKADDNR KIVLISGALNDKRNELKTKK ENDEAELTELLDNIKARVDR IDNIKVRTITATSRERKDAL KYGLSMGKYCLYLEAQELNG SITIDEVHDMSISDMIEKLE HHHHHH

Data sets:
Data typeCount
13C chemical shifts773
15N chemical shifts189
1H chemical shifts1261

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 10 residues - 1190.390 Da.

1   METTYRGLYTYRILECYSVALASPILEASN

Entity 2, unit_2 166 residues - 19081.623 Da.

1   PROSERVALGLULEUVALILEASPGLUTHR
2   CYSARGVALLEUGLUVALARGPROGLNASN
3   LYSASPGLYGLUGLNLEUILESERGLYLEU
4   GLULEULEUASPLYSASNVALGLUASPVAL
5   VALTYRGLULEUILEASNARGSERILESER
6   PHEGLYPHEVALLYSALAASPASPASNARG
7   LYSILEVALLEUILESERGLYALALEUASN
8   ASPLYSARGASNGLULEULYSTHRLYSLYS
9   GLUASNASPGLUALAGLULEUTHRGLULEU
10   LEUASPASNILELYSALAARGVALASPARG
11   ILEASPASNILELYSVALARGTHRILETHR
12   ALATHRSERARGGLUARGLYSASPALALEU
13   LYSTYRGLYLEUSERMETGLYLYSTYRCYS
14   LEUTYRLEUGLUALAGLNGLULEUASNGLY
15   SERILETHRILEASPGLUVALHISASPMET
16   SERILESERASPMETILEGLULYSLEUGLU
17   HISHISHISHISHISHIS

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