BMRB Entry 36516

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PDB ID: 8her
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36516
MolProbity Validation Chart

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Title:
Solution structure of the periplasmic domain of RsgI6 from Clostridium thermocellum
Deposition date:
2022-11-08
Original release date:
2023-05-30
Authors:
Chen, C.; Feng, Y.
Citation:

Citation: Chen, C.; Feng, Y.. "Essential autoproteolysis of bacterial anti-sigma factor RsgI for signal transmembrane transduction"  .

Assembly members:

Assembly members:
Anti-sigma factor, polymer, 10 residues, 1158.325 Da.
Anti-sigma factor, polymer, 166 residues, 18660.010 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 637887   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Acetivibrio thermocellus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Anti-sigma factor: MYAYVGIDIN
Anti-sigma factor: PSIELWINYNNKIAEAKALN GDAETVLEGLELKEKTVAEA VNEIVQKSMELGFISREKEN IILISTACDLKAGEGSENKD VQNKIGQLFDDVNKAVSDLK NSGITTRILNLTLEERESSK EENISMGRYAVYLKAKEQNV NLTIDEIKDADLLELIAKLE HHHHHH

Data sets:
Data typeCount
13C chemical shifts768
15N chemical shifts192
1H chemical shifts1256

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 10 residues - 1158.325 Da.

1   METTYRALATYRVALGLYILEASPILEASN

Entity 2, unit_2 166 residues - 18660.010 Da.

1   PROSERILEGLULEUTRPILEASNTYRASN
2   ASNLYSILEALAGLUALALYSALALEUASN
3   GLYASPALAGLUTHRVALLEUGLUGLYLEU
4   GLULEULYSGLULYSTHRVALALAGLUALA
5   VALASNGLUILEVALGLNLYSSERMETGLU
6   LEUGLYPHEILESERARGGLULYSGLUASN
7   ILEILELEUILESERTHRALACYSASPLEU
8   LYSALAGLYGLUGLYSERGLUASNLYSASP
9   VALGLNASNLYSILEGLYGLNLEUPHEASP
10   ASPVALASNLYSALAVALSERASPLEULYS
11   ASNSERGLYILETHRTHRARGILELEUASN
12   LEUTHRLEUGLUGLUARGGLUSERSERLYS
13   GLUGLUASNILESERMETGLYARGTYRALA
14   VALTYRLEULYSALALYSGLUGLNASNVAL
15   ASNLEUTHRILEASPGLUILELYSASPALA
16   ASPLEULEUGLULEUILEALALYSLEUGLU
17   HISHISHISHISHISHIS

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