BMRB

Biological Magnetic Resonance Data Bank


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BMRB Entry 36528

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36528
MolProbity Validation Chart

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Title: NMR Structure of OsCIE1-Ubox

Deposition date: 2022-12-13 Original release date: 2024-03-25

Authors: Zhang, Y.; Yu, C.; Lan, W.

Citation: Zhang, Y.; Yu, C.; Lan, W.. "Release of a Ubiquitin Brake Activates Pattern Recognition Receptor-mediated Immunity in Rice"  .

Assembly members:
U-box domain-containing protein 12, polymer, 160 residues, 8952.272 Da.

Natural source:   Common Name: Asian cultivated rice   Taxonomy ID: 39947   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Oryza sativa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
U-box domain-containing protein 12: GAMIIPDEFRCPISLELMQD PVIVSSGQTYERSCIQKWLD SGHKTCPKTQQPLSHTSLTP NFVLKSLISQWCEANGIELP

Data sets:
Data typeCount
13C chemical shifts305
15N chemical shifts72
1H chemical shifts539

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1_11
2entity_1_21

Entities:

Entity 1, entity_1_1 160 residues - 8952.272 Da.

1   GLYALAMETILEILEPROASPGLUPHEARG
2   CYSPROILESERLEUGLULEUMETGLNASP
3   PROVALILEVALSERSERGLYGLNTHRTYR
4   GLUARGSERCYSILEGLNLYSTRPLEUASP
5   SERGLYHISLYSTHRCYSPROLYSTHRGLN
6   GLNPROLEUSERHISTHRSERLEUTHRPRO
7   ASNPHEVALLEULYSSERLEUILESERGLN
8   TRPCYSGLUALAASNGLYILEGLULEUPRO

Samples:

sample_1: U-box domain-containing protein 12, [U-13C; U-15N], mM; DTT 2 mM; sodium chloride 100 mM; TRIS 10 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 200 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Sparky, Goddard - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

  • Agilent DD2 800 MHz
  • Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts