BMRB Entry 4045
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4045
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Title: Solution Structure of the C-Terminal Single-Stranded DNA-Binding Domain of Escherichia Coli Topoisomerase I
Deposition date: 1997-07-17 Original release date: 2001-02-15
Authors: Yu, Liping; Zhu, Chang-Xi; Tse-Dinh, Yuk-Ching; Fesik, Stephen
Citation: Yu, Liping; Zhu, Chang-Xi; Tse-Dinh, Yuk-Ching; Fesik, Stephen. "Solution Structure of the C-Terminal Single-Stranded DNA-Binding Domain of Escherichia coli Topoisomerase I" Biochemistry 34, 7622-7628 (1995).
Assembly members:
DNA Topoisomerase I, polymer, 122 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
DNA Topoisomerase I: MNGEVAPPKEDPVPLPELPC
EKSDAYFVLRDGAAGVFLAA
NTFPKSRETRAPLVEELYRF
RDRLPEKLRYLADAPQQDPE
GNKTMVRFSRKTKQQYVSSE
KDGKATGWSAFYVDGKWVEG
KK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 441 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 849 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNA Topoisomerase I | 1 |
Entities:
Entity 1, DNA Topoisomerase I 122 residues - Formula weight is not available
| 1 | MET | ASN | GLY | GLU | VAL | ALA | PRO | PRO | LYS | GLU | ||||
| 2 | ASP | PRO | VAL | PRO | LEU | PRO | GLU | LEU | PRO | CYS | ||||
| 3 | GLU | LYS | SER | ASP | ALA | TYR | PHE | VAL | LEU | ARG | ||||
| 4 | ASP | GLY | ALA | ALA | GLY | VAL | PHE | LEU | ALA | ALA | ||||
| 5 | ASN | THR | PHE | PRO | LYS | SER | ARG | GLU | THR | ARG | ||||
| 6 | ALA | PRO | LEU | VAL | GLU | GLU | LEU | TYR | ARG | PHE | ||||
| 7 | ARG | ASP | ARG | LEU | PRO | GLU | LYS | LEU | ARG | TYR | ||||
| 8 | LEU | ALA | ASP | ALA | PRO | GLN | GLN | ASP | PRO | GLU | ||||
| 9 | GLY | ASN | LYS | THR | MET | VAL | ARG | PHE | SER | ARG | ||||
| 10 | LYS | THR | LYS | GLN | GLN | TYR | VAL | SER | SER | GLU | ||||
| 11 | LYS | ASP | GLY | LYS | ALA | THR | GLY | TRP | SER | ALA | ||||
| 12 | PHE | TYR | VAL | ASP | GLY | LYS | TRP | VAL | GLU | GLY | ||||
| 13 | LYS | LYS |
Samples:
sample_one: DNA Topoisomerase I, [U-15N], 3 mM; dithiothreitol-d10 5 mM; KH2PO4 200 mM
sample_two: DNA Topoisomerase I, [U-13C; U-15N], 3 mM; dithiothreitol-d10 5 mM; KH2PO4 200 mM
sample_conditions_one: pH: 6.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker AMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA14811 BAB35269 BAG76846 BAI25122 BAI30265 |
| EMBL | CAA28164 CAP75818 CAQ31777 CAQ98153 CAR02734 |
| GB | AAA23641 AAC74356 AAG56541 AAN42888 AAN80207 |
| REF | NP_309873 NP_415790 NP_707181 WP_000317025 WP_000513607 |
| SP | P06612 |
| AlphaFold | P06612 |
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