BMRB Entry 4048
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4048
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Title: Solution Structure of the tetrameric minimum transforming domain of p53
Deposition date: 1997-07-28 Original release date: 1999-08-17
Authors: Lee, Weontae; Harvey, Timothy; Yin, Ya; Yau, Patrick; Litchfield, David; Arrowsmith, Cheryl
Citation: Lee, Weontae; Harvey, Timothy; Yin, Ya; Yau, Patrick; Litchfield, David; Arrowsmith, Cheryl. "Solution Structure of the tetrameric minimum transforming domain of p53" Nat. Struct. Biol. 1, 877-890 (1994).
Assembly members:
p53 nuclear phosphoprotein, polymer, 66 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET19b
Entity Sequences (FASTA):
p53 nuclear phosphoprotein: HMSTKRALPNNTSSSPQPKK
KPLDGEYFTLQIRGRERFEM
FRELNEALELKDAQAGKEPG
GSRAHS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 145 |
| 15N chemical shifts | 39 |
| 1H chemical shifts | 262 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit A | 1 |
| 2 | subunit B | 1 |
| 3 | subunit C | 1 |
| 4 | subunit D | 1 |
Entities:
Entity 1, subunit A 66 residues - Formula weight is not available
| 1 | HIS | MET | SER | THR | LYS | ARG | ALA | LEU | PRO | ASN | ||||
| 2 | ASN | THR | SER | SER | SER | PRO | GLN | PRO | LYS | LYS | ||||
| 3 | LYS | PRO | LEU | ASP | GLY | GLU | TYR | PHE | THR | LEU | ||||
| 4 | GLN | ILE | ARG | GLY | ARG | GLU | ARG | PHE | GLU | MET | ||||
| 5 | PHE | ARG | GLU | LEU | ASN | GLU | ALA | LEU | GLU | LEU | ||||
| 6 | LYS | ASP | ALA | GLN | ALA | GLY | LYS | GLU | PRO | GLY | ||||
| 7 | GLY | SER | ARG | ALA | HIS | SER |
Samples:
sample_one: p53 nuclear phosphoprotein 2.0 mM; NaCL 250 mM; Na2HPO4 25 mM; NaN3 0.02%
sample_two: p53 nuclear phosphoprotein, [U-15N], 2.0 mM; NaCL 250 mM; Na2HPO4 25 mM; NaN3 0.02%
sample_three: p53 nuclear phosphoprotein, [U-13C; U-15N], 2.0 mM; NaCL 250 mM; Na2HPO4 25 mM; NaN3 0.02%
sample_conditions_one: pH: 7.0 na; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Varian Unity 600 600 MHz
- Varian Unity +500 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAC16799 BAD96746 BAG35463 BAG59884 BAG60244 |
| EMBL | CAA25652 CAA26306 CAA34420 CAA38095 CAA42626 |
| GB | AAA17994 AAA59987 AAA59988 AAA59989 AAA61211 |
| PRF | 2006287A |
| REF | NP_000537 NP_001040616 NP_001119584 NP_001119587 NP_001119590 |
| SP | P04637 P13481 P56423 P56424 P61260 |
| AlphaFold | P04637 P13481 P56423 P56424 P61260 |
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