BMRB Entry 4075
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4075
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Title: Backbone NMR Assignments and Secondary Structure of the N Terminal Domain of DnaB Helicase from E. coli
Deposition date: 1997-11-25 Original release date: 1998-10-12
Authors: Weigelt, Johan; Miles, Caroline; Dixon, Nicholas; Otting, Gottfried
Citation: Weigelt, Johan; Miles, Caroline; Dixon, Nicholas; Otting, Gottfried. "Backbone NMR Assignments and Secondary Structure of the N Terminal Domain of DnaB Helicase from E. coli" J. Biomol. NMR 11, 233-234 (1998).
Assembly members:
DnaB helicase, polymer, 142 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCM865
Entity Sequences (FASTA):
DnaB helicase: AGNKPFNKQQAEPRERDPQV
AGLKVPPHSIEAEQSVLGGL
MLDNERWDDVAERVVADDFY
TRPHRHIFTEMARLQESGSP
IDLITLAESLERQGQLDSVG
GFAYLAELSKNTPSAANISA
YADIVRERAVVREMISVANE
IA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 253 |
| 15N chemical shifts | 124 |
| 1H chemical shifts | 259 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DnaB-Nterm | 1 |
Entities:
Entity 1, DnaB-Nterm 142 residues - Formula weight is not available
| 1 | ALA | GLY | ASN | LYS | PRO | PHE | ASN | LYS | GLN | GLN | ||||
| 2 | ALA | GLU | PRO | ARG | GLU | ARG | ASP | PRO | GLN | VAL | ||||
| 3 | ALA | GLY | LEU | LYS | VAL | PRO | PRO | HIS | SER | ILE | ||||
| 4 | GLU | ALA | GLU | GLN | SER | VAL | LEU | GLY | GLY | LEU | ||||
| 5 | MET | LEU | ASP | ASN | GLU | ARG | TRP | ASP | ASP | VAL | ||||
| 6 | ALA | GLU | ARG | VAL | VAL | ALA | ASP | ASP | PHE | TYR | ||||
| 7 | THR | ARG | PRO | HIS | ARG | HIS | ILE | PHE | THR | GLU | ||||
| 8 | MET | ALA | ARG | LEU | GLN | GLU | SER | GLY | SER | PRO | ||||
| 9 | ILE | ASP | LEU | ILE | THR | LEU | ALA | GLU | SER | LEU | ||||
| 10 | GLU | ARG | GLN | GLY | GLN | LEU | ASP | SER | VAL | GLY | ||||
| 11 | GLY | PHE | ALA | TYR | LEU | ALA | GLU | LEU | SER | LYS | ||||
| 12 | ASN | THR | PRO | SER | ALA | ALA | ASN | ILE | SER | ALA | ||||
| 13 | TYR | ALA | ASP | ILE | VAL | ARG | GLU | ARG | ALA | VAL | ||||
| 14 | VAL | ARG | GLU | MET | ILE | SER | VAL | ALA | ASN | GLU | ||||
| 15 | ILE | ALA |
Samples:
sample_one: DnaB helicase, [U-13C;U-15N], 1.8 mM; sodium phosphate 20 mM; pefabloc 1 mM; EDTA 1 mM; NaN3 0.02 % w/v
sample_conditions: pH: 6.5; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| BMRB | 4297 |
| PDB | |
| DBJ | BAB38457 BAE78054 BAG66812 BAG79869 BAH61192 |
| EMBL | CAP78509 CAQ34401 CAQ91563 CAR01031 CAR05689 |
| GB | AAA23689 AAA23690 AAC43146 AAC77022 AAG59250 |
| REF | NP_313061 NP_418476 NP_709868 WP_000918354 WP_000918355 |
| SP | P0ACB0 P0ACB1 Q8FB22 Q8X5V3 |
| AlphaFold | P0ACB0 P0ACB1 Q8FB22 Q8X5V3 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts