BMRB Entry 4085
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4085
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Title: Resonance Assignments and Solution Structure of the Second RNA-Binding Domain of Sex-lethal Determined by Multidimensional Heteronuclear Magnetic Resonance PubMed: 7524663
Deposition date: 1997-12-23 Original release date: 2001-02-16
Authors: Lee, Andrew; Kanaar, Roland; Rio, Donald; Wemmer, David
Citation: Lee, Andrew; Kanaar, Roland; Rio, Donald; Wemmer, David. "Resonance Assignments and Solution Structure of the Second RNA-Binding Domain of Sex-lethal Determined by Multidimensional Heteronuclear Magnetic Resonance" Biochemistry 33, 13775-13786 (1994).
Assembly members:
Sex-lethal protein RNA binding domain 2, polymer, 97 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pAR-Sxl-RNAP-2
Entity Sequences (FASTA):
Sex-lethal protein RNA binding domain 2: MSYARPGGESIKDTNLYVTN
LPRTITDDQLDTIFGKYGSI
VQKNILRDKLTGRPRGVAFV
RYNKREEAQEAISALNNVIP
EGGSQPLSVRLAEEHGK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 288 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 608 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sxl-RBD2 | 1 |
Entities:
Entity 1, Sxl-RBD2 97 residues - Formula weight is not available
| 1 | MET | SER | TYR | ALA | ARG | PRO | GLY | GLY | GLU | SER | ||||
| 2 | ILE | LYS | ASP | THR | ASN | LEU | TYR | VAL | THR | ASN | ||||
| 3 | LEU | PRO | ARG | THR | ILE | THR | ASP | ASP | GLN | LEU | ||||
| 4 | ASP | THR | ILE | PHE | GLY | LYS | TYR | GLY | SER | ILE | ||||
| 5 | VAL | GLN | LYS | ASN | ILE | LEU | ARG | ASP | LYS | LEU | ||||
| 6 | THR | GLY | ARG | PRO | ARG | GLY | VAL | ALA | PHE | VAL | ||||
| 7 | ARG | TYR | ASN | LYS | ARG | GLU | GLU | ALA | GLN | GLU | ||||
| 8 | ALA | ILE | SER | ALA | LEU | ASN | ASN | VAL | ILE | PRO | ||||
| 9 | GLU | GLY | GLY | SER | GLN | PRO | LEU | SER | VAL | ARG | ||||
| 10 | LEU | ALA | GLU | GLU | HIS | GLY | LYS |
Samples:
sample_one: Sex-lethal protein RNA binding domain 2, [U-15N], 1.5 – 2.0 mM; NaCL 25 mM; d4-acetic_acid 25 mM; NaN3 0.01%; D20 99.996%
sample_two: Sex-lethal protein RNA binding domain 2, [U-15N], 1.5 – 2.0 mM; NaCL 25 mM; d4-acetic_acid 25 mM; NaN3 0.01%; H20 10%; D20 90%
sample_three: Sex-lethal protein RNA binding domain 2, [U-13C; U-15N], 1.5 – 2.0 mM; NaCL 25 mM; d4-acetic_acid 25 mM; NaN3 0.01%; D20 99.996%
sample_four: Sex-lethal protein RNA binding domain 2, [U-13C; U-15N], 1.5 – 2.0 mM; NaCL 25 mM; d4-acetic_acid 25 mM; NaN3 0.01%; H20 10%; D20 90%
sample_conditions: pH: 5.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker AMX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts