BMRB Entry 4095
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4095
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Title: From Genetic to Structural Characterization of a New Class of RNA-Binding Domain Within the SacY/BglG family of Antiterminator
Deposition date: 1998-01-16 Original release date: 1998-09-04
Authors: Manival, Xavier; Yang, Yinshan; Strub, Marie-paule; Kochoyan, Michel; Steinmetz, Michel; Aymerich, Stephane
Citation: Manival, Xavier; Yang, Yinshan; Strub, Marie-paule; Kochoyan, Michel; Steinmetz, Michel; Aymerich, Stephane. "From Genetic to Structural Characterization of a New Class of RNA-Binding Domain Within the SacY/BglG family of Antiterminator" EMBO J. 16, 5019-5029 (1997).
Assembly members:
sacB mRNA binding domain, polymer, 57 residues, 12992 Da.
Natural source: Common Name: not available Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T
Entity Sequences (FASTA):
sacB mRNA binding domain: GSMKIKRILNHNAIVVKDQN
EEKILLGAGIAFNKKKNDIV
DPSKIEKTFIRKDTPDY
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 53 |
| 1H chemical shifts | 423 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Chain A | 1 |
| 2 | Chain B | 1 |
Entities:
Entity 1, Chain A 57 residues - 12992 Da.
| 1 | GLY | SER | MET | LYS | ILE | LYS | ARG | ILE | LEU | ASN | ||||
| 2 | HIS | ASN | ALA | ILE | VAL | VAL | LYS | ASP | GLN | ASN | ||||
| 3 | GLU | GLU | LYS | ILE | LEU | LEU | GLY | ALA | GLY | ILE | ||||
| 4 | ALA | PHE | ASN | LYS | LYS | LYS | ASN | ASP | ILE | VAL | ||||
| 5 | ASP | PRO | SER | LYS | ILE | GLU | LYS | THR | PHE | ILE | ||||
| 6 | ARG | LYS | ASP | THR | PRO | ASP | TYR |
Samples:
sample_one: sacB mRNA binding domain, [U-15N], 3 mM
sample_conditions: ionic strength: 300 mM; pH: 5.2; temperature: 301 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- . . 600 MHz
Related Database Links:
| BMRB | 4096 |
| PDB | |
| DBJ | BAI87513 BAM55925 BAM59937 GAK81163 |
| EMBL | CAA36720 CAA51569 CAB15868 CCU57104 CEI59651 |
| GB | AAA75336 AEP92902 AFQ59694 AGA22707 AGE65457 |
| REF | NP_391721 WP_003227349 WP_003244589 WP_014478367 WP_015250925 |
| SP | P15401 |
| AlphaFold | P15401 |
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