BMRB Entry 4134

Name: NMR Assignments for Denatured LysN
Published: 1999-06-17

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4134

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Assignments for Denatured LysN

Deposition date:

1998-04-16

Original release date:

1999-06-17

Authors:

Alexandrescu, Andrei; Jaravine, Viktor; Lamour, Francois

Citation:

Citation: Alexandrescu, Andrei; Jaravine, Viktor; Lamour, Francois. "NMR Assignments for denatured LysN" (1999).

Assembly members:

Assembly members:
lysl-tRNA_synthetase, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
lysl-tRNA_synthetase: AEQGIAFPNDFRRDHTSDQL HAEFDGKENEELEALNIEVA VAGRMMTRRIMGKASFVTLQ DVGGRIQLYVARDDLPEGVY NEQFKKWDLGDILGAKGKLF KTKTGELSIHCTELRLLTKA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_one

Data type	Count
13C chemical shifts	219
15N chemical shifts	115
1H chemical shifts	420

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	den-LysN	1

Entities:

Entity 1, den-LysN 120 residues - Formula weight is not available

1	ALA	GLU	GLN	GLY	ILE	ALA	PHE	PRO	ASN	ASP
2	PHE	ARG	ARG	ASP	HIS	THR	SER	ASP	GLN	LEU
3	HIS	ALA	GLU	PHE	ASP	GLY	LYS	GLU	ASN	GLU
4	GLU	LEU	GLU	ALA	LEU	ASN	ILE	GLU	VAL	ALA
5	VAL	ALA	GLY	ARG	MET	MET	THR	ARG	ARG	ILE
6	MET	GLY	LYS	ALA	SER	PHE	VAL	THR	LEU	GLN
7	ASP	VAL	GLY	GLY	ARG	ILE	GLN	LEU	TYR	VAL
8	ALA	ARG	ASP	ASP	LEU	PRO	GLU	GLY	VAL	TYR
9	ASN	GLU	GLN	PHE	LYS	LYS	TRP	ASP	LEU	GLY
10	ASP	ILE	LEU	GLY	ALA	LYS	GLY	LYS	LEU	PHE
11	LYS	THR	LYS	THR	GLY	GLU	LEU	SER	ILE	HIS
12	CYS	THR	GLU	LEU	ARG	LEU	LEU	THR	LYS	ALA

Samples:

sample_one: lysl-tRNA_synthetase, [U-15N; U-13C], 1.5 mM; urea 3 M; H2O 90%; D2O 10%

sample_two: lysl-tRNA_synthetase, [U-15N], 1 mM; urea 3 M; H2O 90%; D2O 10%

sample_conditions_one: pH: 2.8 na; pressure: 1 atm; temperature: 284 K

Experiments:

Name	Sample	Sample state	Sample conditions
NMR spectral acquisition parameters	sample_two	not available	sample_conditions_one
t3 H 599.9094119 146ms . 7000Hz No 1024 complex .	sample_two	not available	sample_conditions_one
t2 C 150.8529083 7.7ms . 8300Hz No 64 complex .	sample_two	not available	sample_conditions_one
t1 N 60.7952139 21ms . 1540Hz No 32 complex .	sample_two	not available	sample_conditions_one
NMR spectral processing parameters	sample_two	not available	sample_conditions_one
D1 H sine-bell 1024 90 Zero-fill 2048	sample_two	not available	sample_conditions_one
D2 C squared-sine-bell 64 70 Zero-fill 128	sample_two	not available	sample_conditions_one
D3 N linear predict 32 to 48 squaredsine-bell 48 70 Zero-fill 64	sample_two	not available	sample_conditions_one
46 ms mix time	sample_one	not available	sample_conditions_one
NMR spectral acquisition parameters	sample_one	not available	sample_conditions_one
t3 H 600.1328206MHz 85ms 46ms 6009.615Hz No 512 complex .	sample_one	not available	sample_conditions_one
t2 N 60.8178387MHz 21ms . 1521Hz No 32 complex .	sample_one	not available	sample_conditions_one
t1 H 600.1328206MHz 21ms . 6009.615Hz No 128 complex .	sample_one	not available	sample_conditions_one
NMR spectral processing parameters	sample_one	not available	sample_conditions_one
3D [1H-15N] TOCSY-HSQC	sample_one	not available	sample_conditions_one
D1 H gm -10 0.1 Zero-fill 1024	sample_one	not available	sample_conditions_one
D2 N lpl 32 to 48 gm -13 0.1 Zero-fill 64	sample_one	not available	sample_conditions_one
D3 H gm -13 0.1 zf 256	sample_one	not available	sample_conditions_one
200 ms mix time	sample_one	not available	sample_conditions_one
t3 H 599.9094183MHz 146ms 200ms 7000Hz No 1024 complex .	sample_one	not available	sample_conditions_one
t2 H 599.9094183MHz 18ms . 7000Hz No 128 complex .	sample_one	not available	sample_conditions_one
t1 N 60.7951139MHz 21ms . 1540Hz No 32 complex .	sample_one	not available	sample_conditions_one
3D [1H-15N] NOESY-HSQC	sample_one	not available	sample_conditions_one
D1 H gm -5 0.1 Zero-fill 2048	sample_one	not available	sample_conditions_one
D2 H gm -7 0.1 Zero-fill 256	sample_one	not available	sample_conditions_one
D3 N lpl 32 to 48 gm -15 0.1 Zero-fill 64	sample_one	not available	sample_conditions_one

PDB	1BBU 1BBW 1KRS 1KRT
DBJ	BAB37185 BAE76955 BAG78677 BAI27164 BAI32202
EMBL	CAP77327 CAQ33202 CAQ90319 CAQ99824 CAR04406
GB	AAA23959 AAA83071 AAC75928 AAG58018 AAN44362
REF	NP_311789 NP_417366 NP_708655 WP_000003059 WP_000003060
SP	B2U0Q7 P0A8N3 P0A8N4 Q0T106 Q31WF2
AlphaFold	B2U0Q7 P0A8N3 P0A8N4 Q0T106 Q31WF2

BMRB Entry 4134

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	ALA	GLU	GLN	GLY	ILE	ALA	PHE	PRO	ASN	ASP
2	PHE	ARG	ARG	ASP	HIS	THR	SER	ASP	GLN	LEU
3	HIS	ALA	GLU	PHE	ASP	GLY	LYS	GLU	ASN	GLU
4	GLU	LEU	GLU	ALA	LEU	ASN	ILE	GLU	VAL	ALA
5	VAL	ALA	GLY	ARG	MET	MET	THR	ARG	ARG	ILE
6	MET	GLY	LYS	ALA	SER	PHE	VAL	THR	LEU	GLN
7	ASP	VAL	GLY	GLY	ARG	ILE	GLN	LEU	TYR	VAL
8	ALA	ARG	ASP	ASP	LEU	PRO	GLU	GLY	VAL	TYR
9	ASN	GLU	GLN	PHE	LYS	LYS	TRP	ASP	LEU	GLY
10	ASP	ILE	LEU	GLY	ALA	LYS	GLY	LYS	LEU	PHE
11	LYS	THR	LYS	THR	GLY	GLU	LEU	SER	ILE	HIS
12	CYS	THR	GLU	LEU	ARG	LEU	LEU	THR	LYS	ALA

1	ALA	GLU	GLN	GLY	ILE	ALA	PHE	PRO	ASN	ASP
2	PHE	ARG	ARG	ASP	HIS	THR	SER	ASP	GLN	LEU
3	HIS	ALA	GLU	PHE	ASP	GLY	LYS	GLU	ASN	GLU
4	GLU	LEU	GLU	ALA	LEU	ASN	ILE	GLU	VAL	ALA
5	VAL	ALA	GLY	ARG	MET	MET	THR	ARG	ARG	ILE
6	MET	GLY	LYS	ALA	SER	PHE	VAL	THR	LEU	GLN
7	ASP	VAL	GLY	GLY	ARG	ILE	GLN	LEU	TYR	VAL
8	ALA	ARG	ASP	ASP	LEU	PRO	GLU	GLY	VAL	TYR
9	ASN	GLU	GLN	PHE	LYS	LYS	TRP	ASP	LEU	GLY
10	ASP	ILE	LEU	GLY	ALA	LYS	GLY	LYS	LEU	PHE
11	LYS	THR	LYS	THR	GLY	GLU	LEU	SER	ILE	HIS
12	CYS	THR	GLU	LEU	ARG	LEU	LEU	THR	LYS	ALA

1	ALA	GLU	GLN	GLY	ILE	ALA	PHE	PRO	ASN	ASP
2	PHE	ARG	ARG	ASP	HIS	THR	SER	ASP	GLN	LEU
3	HIS	ALA	GLU	PHE	ASP	GLY	LYS	GLU	ASN	GLU
4	GLU	LEU	GLU	ALA	LEU	ASN	ILE	GLU	VAL	ALA
5	VAL	ALA	GLY	ARG	MET	MET	THR	ARG	ARG	ILE
6	MET	GLY	LYS	ALA	SER	PHE	VAL	THR	LEU	GLN
7	ASP	VAL	GLY	GLY	ARG	ILE	GLN	LEU	TYR	VAL
8	ALA	ARG	ASP	ASP	LEU	PRO	GLU	GLY	VAL	TYR
9	ASN	GLU	GLN	PHE	LYS	LYS	TRP	ASP	LEU	GLY
10	ASP	ILE	LEU	GLY	ALA	LYS	GLY	LYS	LEU	PHE
11	LYS	THR	LYS	THR	GLY	GLU	LEU	SER	ILE	HIS
12	CYS	THR	GLU	LEU	ARG	LEU	LEU	THR	LYS	ALA