BMRB Entry 4138
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4138
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Title: Proton Resonance Assignments of RC-RNase
Deposition date: 1998-05-06 Original release date: 1998-06-15
Authors: Chen, Chinpan; Hom, Kellie; Huang, Rong-Fong; Chou, Ping-Jung; Laio, You-Di; Huang, Tai-huang
Citation: Chen, Chinpan; Hom, Kellie; Huang, Rong-Fong; Chou, Ping-Jung; Laio, You-Di; Huang, Tai-huang. "The Secondary Structure of a Pyrimidine-Guanine Sequence-Specific Ribonuclease Possessing Cytotoxic Activity from the Oocytes of Rana catesbeiana" J. Biomol NMR 8, 331-344 (1996).
Assembly members:
RC-RNase, polymer, 111 residues, 12440 Da.
Natural source: Common Name: bull-frog Taxonomy ID: 8400 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rana Catesbeiana
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
RC-RNase: XNWATFQQKHIINTPIINCN
TIMDNNIYIVGGQCKRVNTF
IISSATTVKAICTGVINMNV
LSTTRFQLNTCTRTSITPRP
CPYSSRTETNYICVKCENQY
PVHFAGIGRCP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 768 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RC-RNase | 1 |
Entities:
Entity 1, RC-RNase 111 residues - 12440 Da.
| 1 | PCA | ASN | TRP | ALA | THR | PHE | GLN | GLN | LYS | HIS | ||||
| 2 | ILE | ILE | ASN | THR | PRO | ILE | ILE | ASN | CYS | ASN | ||||
| 3 | THR | ILE | MET | ASP | ASN | ASN | ILE | TYR | ILE | VAL | ||||
| 4 | GLY | GLY | GLN | CYS | LYS | ARG | VAL | ASN | THR | PHE | ||||
| 5 | ILE | ILE | SER | SER | ALA | THR | THR | VAL | LYS | ALA | ||||
| 6 | ILE | CYS | THR | GLY | VAL | ILE | ASN | MET | ASN | VAL | ||||
| 7 | LEU | SER | THR | THR | ARG | PHE | GLN | LEU | ASN | THR | ||||
| 8 | CYS | THR | ARG | THR | SER | ILE | THR | PRO | ARG | PRO | ||||
| 9 | CYS | PRO | TYR | SER | SER | ARG | THR | GLU | THR | ASN | ||||
| 10 | TYR | ILE | CYS | VAL | LYS | CYS | GLU | ASN | GLN | TYR | ||||
| 11 | PRO | VAL | HIS | PHE | ALA | GLY | ILE | GLY | ARG | CYS | ||||
| 12 | PRO |
Samples:
sample_one: RC-RNase2 – 3 mM; H2O 90%; D2O 10%; phosphate buffer 50 mM
sample_conditions_one: pH: 3.5; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz