BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4144

Title: 1H, 13C, 15N Sequential Assignment of the RNA Polymerase 'H' Subunit from Methanococcus jannaschii

Deposition date: 1998-05-26 Original release date: 1998-10-05

Authors: Hodach, Meri; Elorante, Jyrki; Kostourou, Vassiliki; Wienzierl, Robert; Matthews, Stephen

Citation: Hodach, Meri; Elorante, Jyrki; Kostourou, Vassiliki; Wienzierl, Robert; Matthews, Stephen. "1H, 13C, 15N Sequential Assignment of the RNA Polymerase 'H' Subunit from Methanococcus jannaschii"  J Biomol. NMR ., .-. (1998).

Assembly members:
RPB5, polymer, 78 residues, Formula weight is not available

Natural source:   Common Name: Methanococcus jannaschii   Taxonomy ID: 2190   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Methanococcus jannaschii

Experimental source:   Production method: recombinant technology   Host organism: Methanoccocus jannaschii   Vector: pGEX-2T

Entity Sequences (FASTA):
RPB5: MKVTDHILVPKHEIVPKEEV EEILKRYNIKIQQLPKIYED DPVIQEIGAKEGDVVRVIRK SPTAGVSIAYRLVIKRII

Data sets:
Data typeCount
13C chemical shifts219
15N chemical shifts68
1H chemical shifts151

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1rpb51

Entities:

Entity 1, rpb5 78 residues - Formula weight is not available

1   METLYSVALTHRASPHISILELEUVALPRO
2   LYSHISGLUILEVALPROLYSGLUGLUVAL
3   GLUGLUILELEULYSARGTYRASNILELYS
4   ILEGLNGLNLEUPROLYSILETYRGLUASP
5   ASPPROVALILEGLNGLUILEGLYALALYS
6   GLUGLYASPVALVALARGVALILEARGLYS
7   SERPROTHRALAGLYVALSERILEALATYR
8   ARGLEUVALILELYSARGILEILE

Samples:

sample_one: RPB5, [U-13C;U-15N], 1.0 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 5.0; temperature: 302 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX-500 500 MHz

Related Database Links:

PDB
GB AAB99042
SP Q58443
AlphaFold Q58443

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts