BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4252

Title: Triple Resonance Assignment for Abl SH(32) and One in the Complex with a Consolidated Ligand

Deposition date: 1998-10-19 Original release date: 2007-07-13

Authors: Xu, Rong; Cahill, Sean; Cowburn, David

Citation: Xu, Rong; Cahill, Sean; Cowburn, David. "Triple resonance-based assignment for Abl SH(32) and its complex with a consolidated ligand"  J. Biomol. NMR 14, 187-188 (1999).

Assembly members:
Src homology domain 3 and 2, polymer, 166 residues, Formula weight is not available
Abl SH3 binding peptide, polymer, 10 residues, Formula weight is not available
Abl SH2 binding peptide, polymer, 6 residues, Formula weight is not available
G6-Kamide linker, polymer, 6 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Src homology domain 3 and 2: GSPGGSLFVALYDFVASGDN TLSITKGEKLRVLGYNHNGE WAEAQTKNGQGWVPSNYITP VNSLEKHSWYHGPVSRNAAE YLLSSGINGSFLVRESESSP GQRSISLRYEGRVYHYRINT ASDGKLYVSSESRFNTLAEL VHHHSTVADGLITTLHYPAP KRGIRD
Abl SH3 binding peptide: PPAYAPPPVP
Abl SH2 binding peptide: PVYENV
G6-Kamide linker: GGGGGG

Data sets:
Data typeCount
13C chemical shifts407
15N chemical shifts151
1H chemical shifts882

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Abl SH(32)1
2consolidated ligand fragment 12
3consolidated ligand fragment 23
4G6-Kamide linker4

Entities:

Entity 1, Abl SH(32) 166 residues - Formula weight is not available

1   GLYSERPROGLYGLYSERLEUPHEVALALA
2   LEUTYRASPPHEVALALASERGLYASPASN
3   THRLEUSERILETHRLYSGLYGLULYSLEU
4   ARGVALLEUGLYTYRASNHISASNGLYGLU
5   TRPALAGLUALAGLNTHRLYSASNGLYGLN
6   GLYTRPVALPROSERASNTYRILETHRPRO
7   VALASNSERLEUGLULYSHISSERTRPTYR
8   HISGLYPROVALSERARGASNALAALAGLU
9   TYRLEULEUSERSERGLYILEASNGLYSER
10   PHELEUVALARGGLUSERGLUSERSERPRO
11   GLYGLNARGSERILESERLEUARGTYRGLU
12   GLYARGVALTYRHISTYRARGILEASNTHR
13   ALASERASPGLYLYSLEUTYRVALSERSER
14   GLUSERARGPHEASNTHRLEUALAGLULEU
15   VALHISHISHISSERTHRVALALAASPGLY
16   LEUILETHRTHRLEUHISTYRPROALAPRO
17   LYSARGGLYILEARGASP

Entity 2, consolidated ligand fragment 1 10 residues - Formula weight is not available

1   PROPROALATYRALAPROPROPROVALPRO

Entity 3, consolidated ligand fragment 2 6 residues - Formula weight is not available

1   PROVALTYRGLUASNVAL

Entity 4, G6-Kamide linker 6 residues - Formula weight is not available

1   GLYGLYGLYGLYGLYGLY

Samples:

sample_one: Src homology domain 3 and 2 0.8 mM

sample_conditions_one: pH: 7.2; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 4251
PDB
DBJ BAC41088 BAD92693 BAG10808
EMBL CAA10376 CAA10377 CAA24781 CAA34438 CAB56204
GB AAA35593 AAA35697 AAA43042 AAA51561 AAA51896
REF NP_001094320 NP_001106174 NP_001193789 NP_001269974 NP_001269975
SP P00519 P00520 P00521 P10447
TPG DAA24240
AlphaFold P00519 P00520 P00521 P10447

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts