BMRB Entry 4253

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4253

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: An Examination of Coaxial Stacking of Helical Stems in a Pseudoknot Motif: the Gene 32 Messenger RNA Pseudoknot of Bacteriophage T2

Deposition date: 1998-10-22 Original release date: 1999-11-29

Authors: Holland, Jason; Hansen, Mark; Du, Zhiua; Hoffman, Dave

Citation: Holland, Jason; Hansen, Mark; Du, Zhiua; Hoffman, Dave. "An Examination of Coaxial Stacking of Helical Stems in a Pseudoknot Motif: the Gene 32 Messenger RNA Pseudoknot of Bacteriophage T2" RNA 5, 257-271 (1999).

Assembly members:
Gene 32 mRNA Pseudoknot of Bacteriophage T2, polymer, 36 residues, Formula weight is not available

Natural source: Common Name: Bacteriophage T2 Taxonomy ID: 10664 Superkingdom: not available Kingdom: not available Genus/species: T4-like phages coliphage T2

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
Gene 32 mRNA Pseudoknot of Bacteriophage T2: GCUGACCAGCUAUGAGGUCA UACAUCGUCAUAGCAC

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment_one

Data type	Count
13C chemical shifts	162
15N chemical shifts	93
1H chemical shifts	221

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	T2 RNA Pseudoknot	1

Entities:

Entity 1, T2 RNA Pseudoknot 36 residues - Formula weight is not available

1

G

C

U

G

A

C

A

G

C

2

U

A

U

G

A

G

U

C

A

3

U

A

C

A

U

C

G

U

C

A

4

U

A

G

C

A

C

Samples:

sample_one: Gene 32 mRNA Pseudoknot of Bacteriophage T2, [U-100% 15N], 10 mg/ul

sample_two: Gene 32 mRNA Pseudoknot of Bacteriophage T2 11 mg/ul

sample_three: Gene 32 mRNA Pseudoknot of Bacteriophage T2 11 mg/ul

sample_conditions_one: pH: 6.4; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H NOESY	sample_one	not available	sample_conditions_one
1H-13C CT-HSQC	sample_one	not available	sample_conditions_one
1H-15N HMBC	sample_one	not available	sample_conditions_one
1H JR-NOESY	sample_one	not available	sample_conditions_one
1H COSY	sample_one	not available	sample_conditions_one
1H TOCSY	sample_one	not available	sample_conditions_one
1H-13C HMQCNOESY	sample_one	not available	sample_conditions_one
1H-13C HCCH-COSY	sample_one	not available	sample_conditions_one

Software:

FELIX v2.0 - chemical shift assignments and NOE crosspeak intensities

NMR spectrometers:

Varian UNITY-Inova . MHz