BMRB Entry 4258
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4258
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Title: 1H Chemical Shift Assignments of the Palindromic 12mer d(TTTGACGCGTCAA)2 (MCB12T) which Contains the Recognition Site for the Yeast Transcription Factor Mbp1.
Deposition date: 1998-10-29 Original release date: 1999-11-23
Authors: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew
Citation: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew. "1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Saccharomyces cerevisiae in Both Its Free and DNA Bound Forms and H1 Assignments of the Free DNA" J. Biomol. NMR 13, 397-398 (1999).
Assembly members:
12MER containing the mlu1 cell cycle box, polymer, 13 residues, Formula weight is not available
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: not available Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: chemically_synthesized
Entity Sequences (FASTA):
12MER containing the mlu1 cell cycle box: TTTGACGCGTCAA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 94 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNA STRAND 1 | 1 |
| 2 | DNA STRAND 2 | 1 |
Entities:
Entity 1, DNA STRAND 1 13 residues - Formula weight is not available
| 1 | DT | DT | DT | DG | DA | DC | DG | DC | DG | DT | ||||
| 2 | DC | DA | DA |
Samples:
sample_one: 12MER containing the mlu1 cell cycle box 1.0 mM; Na phosphate 40 mM; NaCl 100 mM; D2O 10%; H2O 90%
sample_conditions: pH: 7.6; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz