BMRB Entry 4259
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4259
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Title: Sequential Assignment and Solution Secondary Structure of Doubly Labelled Ribonuclease Sa
Deposition date: 1998-10-29 Original release date: 1999-11-23
Authors: Laurents, Douglas; Perez-Canadillas, Jose Manuel; Bruix, Marta; Santoro, Jorge; Schell, David; Hebert, Eric; Pace, C.; Rico, Manuel
Citation: Laurents, Douglas; Perez-Canadillas, Jose Manuel; Santoro, Jorge; Rico, Manuel; Schell, David; Hebert, Eric; Pace, C.; Bruix, Marta. "Sequential Assignment and Solution Secondary Structure of Doubly Labelled Ribonuclease Sa" J. Biomol. NMR 14, 89-90 (1999).
Assembly members:
RNAse-SA, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: S. aureofaciens Taxonomy ID: 1894 Superkingdom: Eubacteria Kingdom: not available Genus/species: Streptomyces aureofaciens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pEH100
Entity Sequences (FASTA):
RNAse-SA: DVSGTVCLSALPPEATDTLN
LIASDGPFPYSQDGVVFQNR
ESVLPTQSYGYYHEYTVITP
GARTRGTRRIITGEATQEDY
YTGDHYATFSLIDQTC
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 183 |
15N chemical shifts | 96 |
1H chemical shifts | 635 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | RNAse-SA | 1 |
Entities:
Entity 1, RNAse-SA 96 residues - Formula weight is not available
1 | ASP | VAL | SER | GLY | THR | VAL | CYS | LEU | SER | ALA | ||||
2 | LEU | PRO | PRO | GLU | ALA | THR | ASP | THR | LEU | ASN | ||||
3 | LEU | ILE | ALA | SER | ASP | GLY | PRO | PHE | PRO | TYR | ||||
4 | SER | GLN | ASP | GLY | VAL | VAL | PHE | GLN | ASN | ARG | ||||
5 | GLU | SER | VAL | LEU | PRO | THR | GLN | SER | TYR | GLY | ||||
6 | TYR | TYR | HIS | GLU | TYR | THR | VAL | ILE | THR | PRO | ||||
7 | GLY | ALA | ARG | THR | ARG | GLY | THR | ARG | ARG | ILE | ||||
8 | ILE | THR | GLY | GLU | ALA | THR | GLN | GLU | ASP | TYR | ||||
9 | TYR | THR | GLY | ASP | HIS | TYR | ALA | THR | PHE | SER | ||||
10 | LEU | ILE | ASP | GLN | THR | CYS |
Samples:
sample_one: RNAse-SA1.5 2.0 mM; H2O 90%; D2O 10%
sample_two: RNAse-SA1.5 2.0 mM; D2O 100%
sample_three: RNAse-SA, [U-15N], 1.5 2.0 mM; H2O 90%; D2O 10%
sample_four: RNAse-SA, [U-15N; 13C], 1.5 2.0 mM; H2O 90%; D2O 10%
sample_conditions_one: pH*: 5.5; temperature: 303.2 K
sample_conditions_two: pH*: 5.5; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
COSY | not available | not available | not available |
NOESY | not available | not available | not available |
TOCSY | not available | not available | not available |
1H-15N TOCSY | not available | not available | not available |
1H-15N NOESY | not available | not available | not available |
HNCA | not available | not available | not available |
HN(CO)CA | not available | not available | not available |
HNCO | not available | not available | not available |
Software:
ANSIG v3.3 - Used for assigning the spectra. Automatic peak picking in 2D
NMR spectrometers:
- Bruker AMX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts