BMRB Entry 4261
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4261
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Hashimoto, Yasuhiro; Toma, Kazunori; Nishikido, Joji; Yamamoto, Keizo; Haneda, Katuji; Inazu, Toshiyuki; Valentine, Kathleen; Opella, Stanley. "Effects of Glycosylation on the Structure and Dynamics of Eel Calcitonin in Micelles and Lipid Bilayers Determined by NMR Spectroscopy" Biochemistry 38, 8377-8384 (1999).
Assembly members:
M6 eel calcitonin, polymer, 32 residues, Formula weight is not available
N-ACETYL-D-GLUCOSAMINE, non-polymer, 221.208 Da.
Natural source: Common Name: eel Taxonomy ID: 7935 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Anguilla not available
Experimental source: Production method: chemically synthesized
Entity Sequences (FASTA):
M6 eel calcitonin: CSNLSTCVLGKLSQELHKLQ
TYPRTDVGAGTP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 211 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | M6 eel calcitonin | 1 |
| 2 | N-acetyl-D-glucosamine | 2 |
Entities:
Entity 1, M6 eel calcitonin 32 residues - Formula weight is not available
| 1 | CYS | SER | ASN | LEU | SER | THR | CYS | VAL | LEU | GLY | ||||
| 2 | LYS | LEU | SER | GLN | GLU | LEU | HIS | LYS | LEU | GLN | ||||
| 3 | THR | TYR | PRO | ARG | THR | ASP | VAL | GLY | ALA | GLY | ||||
| 4 | THR | PRO |
Entity 2, N-acetyl-D-glucosamine - C8 H15 N O6 - 221.208 Da.
| 1 | NAG |
Samples:
sample: M6 eel calcitonin 1 mM
sample_cond_1: pH*: 4.5; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample | not available | sample_cond_1 |
| 2D DQF-COSY | sample | not available | sample_cond_1 |
| 2D TOCSY | sample | not available | sample_cond_1 |
Software:
felix v94 - assignment, peak integration, peak picking
NMR spectrometers:
- Bruker DMX 750 MHz