BMRB Entry 4280
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4280
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Sequence Specific 1H, 13C and 15N Assignment of the Methyl Binding Domain of the Methyl-CpG-binding Protein MeCP2.
Deposition date: 1998-11-23 Original release date: 1999-10-06
Authors: Wakefield, Robert; Smith, Brian; Nan, Xinsheng; Free, Andrew; Soteriou, Alice; Uhrin, Dusan; Bird, Adrian; Barlow, Paul
Citation: Wakefield, Robert; Smith, Brian; Nan, Xinsheng; Free, Andrew; Soteriou, Alice; Uhrin, Dusan; Bird, Adrian; Barlow, Paul. "The solution structure of the domain from MeCP2 that binds to methylated DNA" J. Mol. Biol. 291, 1055-1065 (1999).
Assembly members:
Methyl binding domain of MeCP2, polymer, 92 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eucaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pEH6HMBD
Entity Sequences (FASTA):
Methyl binding domain of MeCP2: ASASPKQRRSIIRDRGPMYD
DPTLPEGWTRKLKQRKSGRS
AGKYDVYLINPQGKAFRSKV
ELIAYFEKVGDTSLDPNDFD
FTVTGRGSGSGC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 291 |
| 15N chemical shifts | 91 |
| 1H chemical shifts | 618 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MBD | 1 |
Entities:
Entity 1, MBD 92 residues - Formula weight is not available
| 1 | ALA | SER | ALA | SER | PRO | LYS | GLN | ARG | ARG | SER | ||||
| 2 | ILE | ILE | ARG | ASP | ARG | GLY | PRO | MET | TYR | ASP | ||||
| 3 | ASP | PRO | THR | LEU | PRO | GLU | GLY | TRP | THR | ARG | ||||
| 4 | LYS | LEU | LYS | GLN | ARG | LYS | SER | GLY | ARG | SER | ||||
| 5 | ALA | GLY | LYS | TYR | ASP | VAL | TYR | LEU | ILE | ASN | ||||
| 6 | PRO | GLN | GLY | LYS | ALA | PHE | ARG | SER | LYS | VAL | ||||
| 7 | GLU | LEU | ILE | ALA | TYR | PHE | GLU | LYS | VAL | GLY | ||||
| 8 | ASP | THR | SER | LEU | ASP | PRO | ASN | ASP | PHE | ASP | ||||
| 9 | PHE | THR | VAL | THR | GLY | ARG | GLY | SER | GLY | SER | ||||
| 10 | GLY | CYS |
Samples:
sample_one: Methyl binding domain of MeCP2, [U-15N; U-13C], 1.0 mM; H2O 90%; D2O 10%
sample_conditions_one: pH: 6.0; temperature: 291 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| BMRB | 4467 |
| PDB | |
| DBJ | BAE34602 BAF82133 BAG73396 |
| EMBL | CAA61599 CAA64331 CAA68001 CAA73190 CAA74577 |
| GB | AAA41584 AAC08757 AAC32737 AAC68880 AAF33023 |
| PIR | S57963 |
| REF | NP_001075448 NP_001104262 NP_001164841 NP_001193481 NP_001244471 |
| SP | P51608 Q00566 Q95LG8 Q9Z2D6 |
| AlphaFold | P51608 Q00566 Q95LG8 Q9Z2D6 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts