BMRB Entry 4282
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4282
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Title: 1H, 15N, and 13C Resonance Assignments for the N-terminal 20 kDa Domain of the DNA Single-Strand Break Repair Protein XRCC1
Deposition date: 1998-12-10 Original release date: 2000-05-03
Authors: Marintchev, Assen; Maciejewski, Mark; Mullen, Gregory
Citation: Marintchev, Assen; Maciejewski, Mark; Mullen, Gregory. "1H, 15N, and 13C Resonance Assignments for the N-terminal 20 kDa Domain of the DNA Single-Strand Break Repair Protein XRCC1" J. Biomol. NMR 13, 393-394 (1999).
Assembly members:
XRCC1 NTD, polymer, 183 residues, 20217 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
XRCC1 NTD: MPEIRLRHVVSCSSQDSTHC
AENLLKADTYRKWRAAKAGE
KTISVVLQLEKEEQIHSVDI
GNDGSAFVEVLVGSSAGGAG
EQDYEVLLVTSSFMSPSESR
SGSNPNRVRMFGPDKLVRAA
AEKRWDRVKIVCSQPYSKDS
PFGLSFVRFHSPPDKDEAEA
PSQKVTVTKLGQFRVKEEEE
SAN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1201 |
| 13C chemical shifts | 766 |
| 15N chemical shifts | 187 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | XRCC1 NTD | 1 |
Entities:
Entity 1, XRCC1 NTD 183 residues - 20217 Da.
| 1 | MET | PRO | GLU | ILE | ARG | LEU | ARG | HIS | VAL | VAL | ||||
| 2 | SER | CYS | SER | SER | GLN | ASP | SER | THR | HIS | CYS | ||||
| 3 | ALA | GLU | ASN | LEU | LEU | LYS | ALA | ASP | THR | TYR | ||||
| 4 | ARG | LYS | TRP | ARG | ALA | ALA | LYS | ALA | GLY | GLU | ||||
| 5 | LYS | THR | ILE | SER | VAL | VAL | LEU | GLN | LEU | GLU | ||||
| 6 | LYS | GLU | GLU | GLN | ILE | HIS | SER | VAL | ASP | ILE | ||||
| 7 | GLY | ASN | ASP | GLY | SER | ALA | PHE | VAL | GLU | VAL | ||||
| 8 | LEU | VAL | GLY | SER | SER | ALA | GLY | GLY | ALA | GLY | ||||
| 9 | GLU | GLN | ASP | TYR | GLU | VAL | LEU | LEU | VAL | THR | ||||
| 10 | SER | SER | PHE | MET | SER | PRO | SER | GLU | SER | ARG | ||||
| 11 | SER | GLY | SER | ASN | PRO | ASN | ARG | VAL | ARG | MET | ||||
| 12 | PHE | GLY | PRO | ASP | LYS | LEU | VAL | ARG | ALA | ALA | ||||
| 13 | ALA | GLU | LYS | ARG | TRP | ASP | ARG | VAL | LYS | ILE | ||||
| 14 | VAL | CYS | SER | GLN | PRO | TYR | SER | LYS | ASP | SER | ||||
| 15 | PRO | PHE | GLY | LEU | SER | PHE | VAL | ARG | PHE | HIS | ||||
| 16 | SER | PRO | PRO | ASP | LYS | ASP | GLU | ALA | GLU | ALA | ||||
| 17 | PRO | SER | GLN | LYS | VAL | THR | VAL | THR | LYS | LEU | ||||
| 18 | GLY | GLN | PHE | ARG | VAL | LYS | GLU | GLU | GLU | GLU | ||||
| 19 | SER | ALA | ASN |
Samples:
sample_1: XRCC1 NTD, [U-15N; U-13C], 2 – 4 mM; DTT 5 mM
Ex-cond: pH: 6.8; temperature: 298 K; ionic strength: 0.4 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | not available | not available | not available |
| 2D 1H-1H TOCSY | not available | not available | not available |
| 2D 1H-13C HSQC | not available | not available | not available |
| 2D 1H-15N HSQC | not available | not available | not available |
| 2D (HB)CB(CGCD)HD | not available | not available | not available |
| 3D 1H-1H-15N NOESY | not available | not available | not available |
| 3D 13C-1H-1H NOESY | not available | not available | not available |
| 3D 1H-1H-15N TOCSY | not available | not available | not available |
| 3D 1H-13C-1H HCCH-TOCSY | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D HN(CO)CA | not available | not available | not available |
| 3D HCC(CO)NH | not available | not available | not available |
| 3D CC(CO)NH | not available | not available | not available |
Software:
FELIX v97 - data processing
XEASY v1.3.13 - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92018 BAG10574 BAG37732 BAG57023 |
| EMBL | CAG33009 |
| GB | AAA63270 AAH23593 AAM34791 EAW57201 EAW57202 |
| REF | NP_006288 XP_001100256 XP_002829368 XP_003281242 XP_003799533 |
| SP | P18887 |
| AlphaFold | P18887 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts