BMRB Entry 4289
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4289
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Title: 1H and 15N Chemical-Shift Assignments of a Carboxy-Terminal Functional Domain of the Bacteriophage P22 Scaffolding Protein
Deposition date: 1998-12-29 Original release date: 2001-03-02
Authors: Sun, Yahong; Krishna, N.
Citation: Sun, Yahong; Krishna, N.. "1H and 15N Chemical-Shift Assignments of a Carboxy-Terminal Functional Domain of the Bacteriophage P22 Scaffolding Protein" Magn. Reson. Chem. 37, 602-604 (1999).
Assembly members:
P22 scaffolding protein, polymer, 67 residues, Formula weight is not available
Natural source: Common Name: bacteriophage P22 Taxonomy ID: 10754 Superkingdom: not available Kingdom: not available Genus/species: not available bacteriophage P22
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
P22 scaffolding protein: ALTRLSERLTLKPRGKQISS
APHADQPITGDVSAANKDAI
RKQMDAAASKGDVETYRKLK
AKLKGIR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 424 |
| 15N chemical shifts | 62 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C-terminal P22 scaffolding protein | 1 |
Entities:
Entity 1, C-terminal P22 scaffolding protein 67 residues - Formula weight is not available
| 1 | ALA | LEU | THR | ARG | LEU | SER | GLU | ARG | LEU | THR | ||||
| 2 | LEU | LYS | PRO | ARG | GLY | LYS | GLN | ILE | SER | SER | ||||
| 3 | ALA | PRO | HIS | ALA | ASP | GLN | PRO | ILE | THR | GLY | ||||
| 4 | ASP | VAL | SER | ALA | ALA | ASN | LYS | ASP | ALA | ILE | ||||
| 5 | ARG | LYS | GLN | MET | ASP | ALA | ALA | ALA | SER | LYS | ||||
| 6 | GLY | ASP | VAL | GLU | THR | TYR | ARG | LYS | LEU | LYS | ||||
| 7 | ALA | LYS | LEU | LYS | GLY | ILE | ARG |
Samples:
sample_1: P22 scaffolding protein, [U-99% 15N], 1.1 mM; NaCl 25 mM; phosphate 50 mM; EDTA 2 mM
sample_2: P22 scaffolding protein 1.1 mM; NaCl 25 mM; phosphate 50 mM; EDTA 2 mM
Ex-cond_1: pH: 4.4; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | not available | not available | Ex-cond_1 |
| TOCSY | not available | not available | Ex-cond_1 |
| DQF-COSY | not available | not available | Ex-cond_1 |
| 1H-15N NOESY-HSQC | not available | not available | Ex-cond_1 |
| 1H-15N TOCSY-HMQC | not available | not available | Ex-cond_1 |
Software:
FELIX v97.2 -
NMR spectrometers:
- Bruker Avance 600 MHz
- Varian INOVA 600 MHz
- Bruker AM 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD15214 BAF80719 BAG12602 BAJ35316 BAP06069 |
| EMBL | CAR60453 CBG23390 CBY96613 CCR48949 CCT11723 |
| GB | AAA72962 AAF75046 AAL15526 AAM81388 AAX21428 |
| REF | NP_720328 WP_000433851 WP_000433852 WP_000433853 WP_000433854 |
| SP | P26748 |
| TPG | DAA00986 |
| AlphaFold | P26748 |
Download HSQC peak lists in one of the following formats:
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