BMRB Entry 4290
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4290
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Title: Sequence-specific 1H Assignment of Antimicrobial Protein Tachycitin. PubMed: 10770921
Deposition date: 1999-01-04 Original release date: 2000-10-30
Authors: Suetake, Tetsuya; Tsuda, Sakae; Kawabata, Shun-ichiro; Kawano, Keiichi; Miura, Kazunori; Iwanaga, Sadaaki; Hikichi, Kunio; Nitta, Katsutoshi; Keiichi, Kawano
Citation: Suetake, Tetsuya; Tsuda, Sakae; Kawabata, Shun-ichiro; Kawano, Keiichi; Miura, Kazunori; Iwanaga, Sadaaki; Hikichi, Kunio; Nitta, Katsutoshi; Keiichi, Kawano. "Chitin-binding Proteins in Invertebrates and Plants Comprise a Common Chitin-binding Structural Motif" J. Biol. Chem. 275, 17929-17932 (2000).
Assembly members:
tachycitin, polymer, 73 residues, 8364 Da.
Natural source: Common Name: Japanese horseshoe crab Taxonomy ID: 6853 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Tachypleus tridentatus
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
tachycitin: YLAFRCGRYSPCLDDGPNVN
LYSCCSFYNCHKCLARLENC
PKGLHYNAYLKVCDWPSKAG
CTSVNKECHLWKT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 496 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | tachycitin | 1 |
Entities:
Entity 1, tachycitin 73 residues - 8364 Da.
| 1 | TYR | LEU | ALA | PHE | ARG | CYS | GLY | ARG | TYR | SER | ||||
| 2 | PRO | CYS | LEU | ASP | ASP | GLY | PRO | ASN | VAL | ASN | ||||
| 3 | LEU | TYR | SER | CYS | CYS | SER | PHE | TYR | ASN | CYS | ||||
| 4 | HIS | LYS | CYS | LEU | ALA | ARG | LEU | GLU | ASN | CYS | ||||
| 5 | PRO | LYS | GLY | LEU | HIS | TYR | ASN | ALA | TYR | LEU | ||||
| 6 | LYS | VAL | CYS | ASP | TRP | PRO | SER | LYS | ALA | GLY | ||||
| 7 | CYS | THR | SER | VAL | ASN | LYS | GLU | CYS | HIS | LEU | ||||
| 8 | TRP | LYS | THR |
Samples:
sample_one: tachycitin1.0 – 2.0 mM
sample_conditions_one: pH: 4.2; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H DQF-COSY | not available | not available | not available |
| 2D 1H-1H TOCSY | not available | not available | not available |
| 2D 1H-1H NOESY | not available | not available | not available |
Software:
Pipp v4.0.4 - peak picking
NMR spectrometers:
- JEOL Alpha 600 MHz
- JEOL Alpha 500 MHz