BMRB Entry 4295
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4295
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Title: Titin Module A71 from Human Cardiac Muscle
Deposition date: 1999-01-18 Original release date: 1999-02-12
Authors: Muhle-Goll, C.; Pastore, A.; Nilges, M.
Citation: Muhle-Goll, C.; Pastore, A.; Nilges, M.. "The 3D Structure of a Type I Module from Titin: a Prototype of Intracellular Fibronectin Type III Domains" Structure 6, 1291-1302 (1998).
Assembly members:
Titin type I domain, polymer, 112 residues, 12458 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: not available Kingdom: Eukaryota Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLysS
Entity Sequences (FASTA):
Titin type I domain: MHHHHHHSSPIDPPGKPVPL
NITRHTVTLKWAKPEYTGGF
KITSYIVEKRDLPNGRWLKA
NFSNILENEFTVSGLTEDAA
YEFRVIAKNAAGAISPPSEP
SDAITCRDDVEA
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 101 |
| 1H chemical shifts | 731 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Titin_type_I_domain-A71 | 1 |
Entities:
Entity 1, Titin_type_I_domain-A71 112 residues - 12458 Da.
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | SER | SER | PRO | ||||
| 2 | ILE | ASP | PRO | PRO | GLY | LYS | PRO | VAL | PRO | LEU | ||||
| 3 | ASN | ILE | THR | ARG | HIS | THR | VAL | THR | LEU | LYS | ||||
| 4 | TRP | ALA | LYS | PRO | GLU | TYR | THR | GLY | GLY | PHE | ||||
| 5 | LYS | ILE | THR | SER | TYR | ILE | VAL | GLU | LYS | ARG | ||||
| 6 | ASP | LEU | PRO | ASN | GLY | ARG | TRP | LEU | LYS | ALA | ||||
| 7 | ASN | PHE | SER | ASN | ILE | LEU | GLU | ASN | GLU | PHE | ||||
| 8 | THR | VAL | SER | GLY | LEU | THR | GLU | ASP | ALA | ALA | ||||
| 9 | TYR | GLU | PHE | ARG | VAL | ILE | ALA | LYS | ASN | ALA | ||||
| 10 | ALA | GLY | ALA | ILE | SER | PRO | PRO | SER | GLU | PRO | ||||
| 11 | SER | ASP | ALA | ILE | THR | CYS | ARG | ASP | ASP | VAL | ||||
| 12 | GLU | ALA |
Samples:
sample_one: Titin type I domain, [U-90% 15N], 1.2 mM; NaCl 50 mM
sample_conditions: pH: 5.6 na; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| HSQC | not available | not available | not available |
| 3D NOESY-HSQC | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker AMX600 600 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts