BMRB Entry 4303
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4303
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Title: Chemical Shift Assignment of Rb. sphearoides LH1b
Deposition date: 1999-02-03 Original release date: 2000-12-14
Authors: Conroy, Matthew; Westerhuis, Willem; Parkes-Loach, Pamela; Loach, Paul; Hunter, C; Williamson, Micheal
Citation: Conroy, Matthew; Westerhuis, Willem; Parkes-Loach, Pamela; Loach, Paul; Hunter, C.; Williamson, Micheal. "The Solution Structure of Rhodobacter sphaeroides LH1beta Reveals Two Helical Domains Separated by a More Flexible Region: Structural Consequences for the LH1 Complex" J. Mol. Biol. 298, 83-94 (2000).
Assembly members:
LH1 beta, polymer, 48 residues, 5455 Da.
Natural source: Common Name: Rhodobacter sphaeroides Taxonomy ID: 1063 Superkingdom: Eubacteria Kingdom: not available Genus/species: Rhodobacter sphaeroides
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
LH1 beta: ADKSDLGYTGLTDEQAQELH
SVYMSGLWLFSAVAIVAHLA
VYIWRPWF
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 342 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LH1 beta | 1 |
Entities:
Entity 1, LH1 beta 48 residues - 5455 Da.
| 1 | ALA | ASP | LYS | SER | ASP | LEU | GLY | TYR | THR | GLY | ||||
| 2 | LEU | THR | ASP | GLU | GLN | ALA | GLN | GLU | LEU | HIS | ||||
| 3 | SER | VAL | TYR | MET | SER | GLY | LEU | TRP | LEU | PHE | ||||
| 4 | SER | ALA | VAL | ALA | ILE | VAL | ALA | HIS | LEU | ALA | ||||
| 5 | VAL | TYR | ILE | TRP | ARG | PRO | TRP | PHE |
Samples:
sample_1: LH1 beta0.5 – 1.5 mM; chloroform 50 % v/v; methanol-d3 50 % v/v
condition_1: pH: .; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| COSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| TOCSY | not available | not available | not available |
Software:
FELIX v97 -
NMR spectrometers:
- Bruker DRX 500 MHz