BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4325

Title: Characterization of Monomeric and Dimeric B-domain of Staphylococcal Protein A.

Deposition date: 1999-03-22 Original release date: 1999-09-13

Authors: Karimi, Afshin; Matsumura, Masazumi; Wright, Peter; Dyson, H.

Citation: Karimi, Afshin; Matsumura, Masazumi; Wright, Peter; Dyson, H.. "Characterization of Monomeric and Dimeric B-domain of Staphylococcal Protein A."  J. Pept. Res. 54, 344-352 (1999).

Assembly members:
dimer of B domain of protein A, polymer, 123 residues, 13000 Da.

Natural source:   Common Name: golden staph.   Taxonomy ID: 1280   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: vendor

Entity Sequences (FASTA):
dimer of B domain of protein A: MADNKFNKEQQNAFYEILHL PNLNEEQRNGFIQSLKDDPS QSANLLAEAKKLNDAQAPKA DNKFNKEQQNAFYEILHLPN LNEEQRNGFIQSLKDDPSQS ANLLAEAKKLNDAQAPKADN KGS

Data sets:
Data typeCount
1H chemical shifts845

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dimeric B domain of protein A one1
2dimeric B domain of protein A two1

Entities:

Entity 1, dimeric B domain of protein A one 123 residues - 13000 Da.

1   METALAASPASNLYSPHEASNLYSGLUGLN
2   GLNASNALAPHETYRGLUILELEUHISLEU
3   PROASNLEUASNGLUGLUGLNARGASNGLY
4   PHEILEGLNSERLEULYSASPASPPROSER
5   GLNSERALAASNLEULEUALAGLUALALYS
6   LYSLEUASNASPALAGLNALAPROLYSALA
7   ASPASNLYSPHEASNLYSGLUGLNGLNASN
8   ALAPHETYRGLUILELEUHISLEUPROASN
9   LEUASNGLUGLUGLNARGASNGLYPHEILE
10   GLNSERLEULYSASPASPPROSERGLNSER
11   ALAASNLEULEUALAGLUALALYSLYSLEU
12   ASNASPALAGLNALAPROLYSALAASPASN
13   LYSGLYSER

Samples:

sample_1: dimer of B domain of protein A1.0 – 4.0 mM

cond-1: pH: 5.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H 2QF COSYnot availablenot availablenot available
1H NOESYnot availablenot availablenot available
1H TOCSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker AMX 600 MHz

Related Database Links:

PDB
EMBL CUD36484
GB ACL54840 EHS72728 EIK36223 EUN97074 EUO28860
REF WP_031792806 WP_031794720 WP_033863190 WP_049280869 WP_049307830