BMRB Entry 4331
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4331
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Title: Backbone 1H, 13C, and 15N Resonance Assignments of Streptomyces Subtilisin Inhibitor
Deposition date: 1999-04-12 Original release date: 1999-05-24
Authors: Sasakawa, Hiroaki; Tamura, Atsuo; Taguchi, Seiichi; Akasaka, Kazuyuki; Miyake, Yoko; Kainosho, Masatsune
Citation: Sasakawa, Hiroaki; Tamura, Atsuo; Taguchi, Seiichi; Akasaka, Kazuyuki; Miyake, Yoko; Kainosho, Masatsune. "Backbone 1H, 13C, and 15N Resonance Assignments of Streptomyces Subtilisin Inhibitor" J. Biomol. NMR ., .-. (1999).
Assembly members:
Streptomyces subtilisin inhibitor, polymer, 113 residues, 11500 Da.
Natural source: Common Name: S. albogriseolus Taxonomy ID: 1887 Superkingdom: Eubacteria Kingdom: not available Genus/species: Streptomyces albogriseolus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Streptomyces subtilisin inhibitor: GAPSALYAPSALVLTVGKGV
SATTAAPERAVTLTCAPGPS
GTHPAAGSACADLAAVGGDL
NALTRGEDVMCPMVYDPVLL
TVDGVWQGKRVSYERVFSNE
CEMNAHGSSVFAF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 198 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 98 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SSI subunit 1 | 1 |
| 2 | SSI subunit 2 | 1 |
Entities:
Entity 1, SSI subunit 1 113 residues - 11500 Da.
| 1 | GLY | ALA | PRO | SER | ALA | LEU | TYR | ALA | PRO | SER | ||||
| 2 | ALA | LEU | VAL | LEU | THR | VAL | GLY | LYS | GLY | VAL | ||||
| 3 | SER | ALA | THR | THR | ALA | ALA | PRO | GLU | ARG | ALA | ||||
| 4 | VAL | THR | LEU | THR | CYS | ALA | PRO | GLY | PRO | SER | ||||
| 5 | GLY | THR | HIS | PRO | ALA | ALA | GLY | SER | ALA | CYS | ||||
| 6 | ALA | ASP | LEU | ALA | ALA | VAL | GLY | GLY | ASP | LEU | ||||
| 7 | ASN | ALA | LEU | THR | ARG | GLY | GLU | ASP | VAL | MET | ||||
| 8 | CYS | PRO | MET | VAL | TYR | ASP | PRO | VAL | LEU | LEU | ||||
| 9 | THR | VAL | ASP | GLY | VAL | TRP | GLN | GLY | LYS | ARG | ||||
| 10 | VAL | SER | TYR | GLU | ARG | VAL | PHE | SER | ASN | GLU | ||||
| 11 | CYS | GLU | MET | ASN | ALA | HIS | GLY | SER | SER | VAL | ||||
| 12 | PHE | ALA | PHE |
Samples:
sample_one: Streptomyces subtilisin inhibitor, [U-13C; U-15N], mM; phosphate 25 mM
sample_conditions: pH: 6.3 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCO | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts