BMRB Entry 4357
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4357
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Title: NMR studies of the Pbx1 TALE Homeodomain Protein free in Solution and bound to DNA : Proposal for a Mechanism of HoxB1-Pbx1-DNA Complex Assembly
Deposition date: 1999-06-14 Original release date: 1999-11-30
Authors: Jabet, Carole; Gitti, Rossitza; Summers, Michael; Wolberger, Cynthia
Citation: Jabet, Carole; Gitti, Rossitza; Summers, Michael; Wolberger, Cynthia. "NMR studies of the Pbx1 TALE Homeodomain Protein free in Solution and bound to DNA : Proposal for a Mechanism of HoxB1-Pbx1-DNA Complex Assembly" J. Mol. Biol. 291, 521-530 (1999).
Assembly members:
Pbx1, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Pbx1: ARRKRRNFNKQATEILNEYF
YSHLSNPYPSEEAKEELAKK
CGITVSQVSNWFGNKRIRYK
KNIGKFQEEANIYAAKTAVT
ATNVSAH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 55 |
| 15N chemical shifts | 55 |
| 1H chemical shifts | 55 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pbx1-HD-short | 1 |
Entities:
Entity 1, Pbx1-HD-short 87 residues - Formula weight is not available
| 1 | ALA | ARG | ARG | LYS | ARG | ARG | ASN | PHE | ASN | LYS | ||||
| 2 | GLN | ALA | THR | GLU | ILE | LEU | ASN | GLU | TYR | PHE | ||||
| 3 | TYR | SER | HIS | LEU | SER | ASN | PRO | TYR | PRO | SER | ||||
| 4 | GLU | GLU | ALA | LYS | GLU | GLU | LEU | ALA | LYS | LYS | ||||
| 5 | CYS | GLY | ILE | THR | VAL | SER | GLN | VAL | SER | ASN | ||||
| 6 | TRP | PHE | GLY | ASN | LYS | ARG | ILE | ARG | TYR | LYS | ||||
| 7 | LYS | ASN | ILE | GLY | LYS | PHE | GLN | GLU | GLU | ALA | ||||
| 8 | ASN | ILE | TYR | ALA | ALA | LYS | THR | ALA | VAL | THR | ||||
| 9 | ALA | THR | ASN | VAL | SER | ALA | HIS |
Samples:
Pbx1-HD_sample_1: Pbx1, [U-90% 15N], 1 mM
Pbx1-HD_sample_2: Pbx1, [U-90% 15N], 1 mM
sample_conditions_1: pH: 7 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
Software:
NMRPipe - data processing
NMRView - peak assignments
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DMX 600 MHz
Related Database Links:
| BMRB | 4358 4359 |
| PDB | |
| DBJ | BAA96135 BAA96136 BAB83538 BAF84104 BAG10741 |
| GB | AAA21832 AAA36484 AAA36764 AAA60031 AAB71191 |
| PIR | B33061 |
| REF | NP_001094151 NP_001128334 NP_001179697 NP_001191890 NP_001191892 |
| SP | P40424 P41778 |
| TPG | DAA32038 |
| AlphaFold | P40424 P41778 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts