BMRB Entry 4374
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4374
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Title: Mutational and structural analyses of the ribonucleotide reductase inhibitor sml1 define its rnr1 interaction domain whose inactivation allows suppression of mec1 and rad53 lethality
Deposition date: 1999-08-05 Original release date: 2002-04-08
Authors: Zao, X.; Goergieva, B.; Chabes, Andrej; Domkin, Vladimir; Ippel, Hans; Schleucher, Juergen; Wijmenga, Sybren; Thelander, Lars; Rothstein, R.
Citation: Zao, X.; Goergieva, B.; Chabes, Andrej; Domkin, Vladimir; Ippel, Hans; Schleucher, Juergen; Wijmenga, Sybren; Thelander, Lars; Rothstein, R.. "Mutational and structural analyses of the ribonucleotide reductase inhibitor sml1 define its rnr1 interaction domain whose inactivation allows suppression of mec1 and rad53 lethality" Mol. Cell. Biol. 20, 9076-9083 (2000).
Assembly members:
Sml1, polymer, 104 residues, 11834.19 Da.
Natural source: Common Name: yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: not available Genus/species: not available Saccharomyces
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Sml1: MQNSQDYFYAQNRCQQQQAP
STLRTVTMAEFRRVPLPPMA
EVPMLSTQNSMGSSASASAS
SLEMWEKDLEERLNSIDHDM
NNNKFGSGELKSMFNQGKVE
EMDF
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 389 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 641 |
| coupling constants | 97 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sml1 protein | 1 |
Entities:
Entity 1, Sml1 protein 104 residues - 11834.19 Da.
| 1 | MET | GLN | ASN | SER | GLN | ASP | TYR | PHE | TYR | ALA | ||||
| 2 | GLN | ASN | ARG | CYS | GLN | GLN | GLN | GLN | ALA | PRO | ||||
| 3 | SER | THR | LEU | ARG | THR | VAL | THR | MET | ALA | GLU | ||||
| 4 | PHE | ARG | ARG | VAL | PRO | LEU | PRO | PRO | MET | ALA | ||||
| 5 | GLU | VAL | PRO | MET | LEU | SER | THR | GLN | ASN | SER | ||||
| 6 | MET | GLY | SER | SER | ALA | SER | ALA | SER | ALA | SER | ||||
| 7 | SER | LEU | GLU | MET | TRP | GLU | LYS | ASP | LEU | GLU | ||||
| 8 | GLU | ARG | LEU | ASN | SER | ILE | ASP | HIS | ASP | MET | ||||
| 9 | ASN | ASN | ASN | LYS | PHE | GLY | SER | GLY | GLU | LEU | ||||
| 10 | LYS | SER | MET | PHE | ASN | GLN | GLY | LYS | VAL | GLU | ||||
| 11 | GLU | MET | ASP | PHE |
Samples:
sample_one: Sml1 0.6 mM; H2O 90%; D2O 10%
sample_2: Sml1, [U-15N], 0.4 mM; H2O 90%; D2O 10%; sodium-phosphate buffer 25 mM; NaCl 25 mM; Dithio-threitol 10 mM
sample_3: Sml1, [U-13C; U-15N], 0.6 mM; H2O 90%; D2O 10%
conditions_1: ionic strength: 50 mM; pH: 6.9; pressure: 1 bar; temperature: 275 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| DBJ | GAA25391 |
| EMBL | CAA86717 CAY81764 |
| GB | AAS56287 AHY76409 AJP40654 AJS61834 AJS62260 |
| REF | NP_013653 |
| SP | Q04964 |
| TPG | DAA09840 |
| AlphaFold | Q04964 |
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